PMID- 20125114
OWN - NLM
STAT- MEDLINE
DCOM- 20100928
LR  - 20180822
IS  - 1440-1711 (Electronic)
IS  - 0818-9641 (Linking)
VI  - 88
IP  - 5
DP  - 2010 Jul
TI  - TLR4: the receptor bridging Acanthamoeba challenge and intracellular inflammatory 
      responses in human corneal cell lines.
PG  - 529-36
LID - 10.1038/icb.2010.6 [doi]
AB  - Acanthamoeba keratitis (AK) is a painful, vision-threatening infection caused by 
      pathogenic strains of the protozoan, Acanthamoeba. Toll-like receptors (TLRs), 
      which are important components of innate immunity, have an important role in the 
      detection of foreign pathogens and the signaling cascades in host cells. However, 
      no report on the interaction between Acanthamoeba and TLR has been found. In this 
      study we analyzed the role of the TLR and its signaling pathway in human 
      telomerase-immortalized corneal epithelial cells (HUCLs) and stromal fibroblasts 
      (THSFs) challenged by Acanthamoeba. We show that the expressions of TLR4, myeloid 
      differentiation protein 88 (MyD88), nuclear factor (NF)-kappaB, phospho-IkappaB, 
      phospho-extracellular signal-regulated kinases 1/2 (p-Erk1/2) and the 
      inflammatory cytokines interleukin (IL)-8, tumor necrosis factor (TNF)-alpha and 
      interferon (IFN)-beta were significantly increased in Acanthamoeba-treated cells. 
      Pretreatment with anti-TLR antibodies or the specific inhibitors pyrrolidine 
      dithiocarbamate (PDTC) (for the NF-kappaB pathway) and U0126 (for the ERK 
      pathway) was conducted. It was found that anti-TLR4 antibody attenuated the 
      production of cytokines induced by Acanthamoeba infection. PDTC inhibited the 
      production of IL-8 and TNF-alpha whereas U0126 inhibited the synthesis of 
      IFN-beta. Thus, TLR4 is a receptor for Acanthamoeba and exerts an effect through 
      TLR4-MyD88-NF-kappaB and TLR4-ERK1/2 pathways to induce the secretion of 
      cytokines in human corneal cell lines challenged by Acanthamoeba.
FAU - Ren, Meiyu
AU  - Ren M
AD  - Department of Ophthalmology, Qilu Hospital, Shandong University, Jinan, China.
FAU - Gao, Li
AU  - Gao L
FAU - Wu, Xinyi
AU  - Wu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100202
PL  - United States
TA  - Immunol Cell Biol
JT  - Immunology and cell biology
JID - 8706300
RN  - 0 (Cytokines)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Acanthamoeba/immunology
MH  - Acanthamoeba Keratitis/*immunology/metabolism
MH  - Blotting, Western
MH  - Cell Line
MH  - Cytokines/biosynthesis/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelium, Corneal/*immunology/metabolism/microbiology
MH  - Humans
MH  - Inflammation/*immunology/metabolism/microbiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/immunology
MH  - Toll-Like Receptor 4/*immunology/metabolism
EDAT- 2010/02/04 06:00
MHDA- 2010/09/30 06:00
CRDT- 2010/02/04 06:00
PHST- 2010/02/04 06:00 [entrez]
PHST- 2010/02/04 06:00 [pubmed]
PHST- 2010/09/30 06:00 [medline]
AID - icb20106 [pii]
AID - 10.1038/icb.2010.6 [doi]
PST - ppublish
SO  - Immunol Cell Biol. 2010 Jul;88(5):529-36. doi: 10.1038/icb.2010.6. Epub 2010 Feb 
      2.