PMID- 20127431
OWN - NLM
STAT- MEDLINE
DCOM- 20120113
LR  - 20211020
IS  - 1473-4230 (Electronic)
IS  - 1473-4222 (Linking)
VI  - 10
IP  - 3
DP  - 2011 Sep
TI  - Neurofilament heavy chain expression reveals a unique parasagittal stripe 
      topography in the mouse cerebellum.
PG  - 409-21
LID - 10.1007/s12311-010-0156-y [doi]
AB  - Despite the general uniformity in cellular composition of the adult cerebellum 
      (Cb), the expression of proteins such as ZebrinII/AldolaseC and the small heat 
      shock protein HSP25 reveal striking patterns of parasagittal Purkinje cell (PC) 
      stripes. Based on differences in the stripe configuration within subsets of 
      lobules, the Cb can be further divided into four anterior-posterior transverse 
      zones: anterior zone (AZ) = lobules I-V, central zone (CZ) = lobules VI-VII, 
      posterior zone (PZ) = lobules VIII and anterior IX, and the nodular zone (NZ) = 
      lobules posterior IX-X. Here we used whole-mount and tissue section 
      immunohistochemistry to show that neurofilament heavy chain (NFH) expression 
      alone divides all lobules of the mouse Cb into a complex series of parasagittal 
      stripes of PCs. We revealed that the striped pattern of NFH in the vermis of the 
      AZ and PZ was complementary to ZebrinII and phospholipase C ss3 (PLCss3), and 
      corresponded to phospholipase C ss4 (PLCss4). In the CZ and NZ the stripe pattern 
      of NFH was complementary to HSP25 and corresponded to PLCss3. The boundaries of 
      the NFH stripes were not always sharply delineated. Instead, a gradual decrease 
      in NFH expression was observed toward the edges of particular stripes, resulting 
      in domains comprised of overlapping expression patterns. Furthermore, the 
      terminal field distributions of mossy and climbing fibers had a complex but 
      consistent topographical alignment with NFH stripes. In summary, NFH expression 
      reveals an exquisite level of Cb stripe complexity that respects the transverse 
      zone divisions and delineates an intricately patterned target field for Cb 
      afferents.
FAU - Demilly, Adrien
AU  - Demilly A
AD  - Dominick P. Purpura Department of Neuroscience, Albert Einstein College of 
      Medicine, Bronx, NY 10461, USA.
FAU - Reeber, Stacey L
AU  - Reeber SL
FAU - Gebre, Samrawit A
AU  - Gebre SA
FAU - Sillitoe, Roy V
AU  - Sillitoe RV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cerebellum
JT  - Cerebellum (London, England)
JID - 101089443
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Hsbp1 protein, mouse)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurofilament Proteins)
RN  - 108688-71-7 (neurofilament protein H)
RN  - EC 3.1.4.11 (Phospholipase C beta)
SB  - IM
MH  - Animals
MH  - Cerebellum/*anatomy & histology/*metabolism
MH  - Heat-Shock Proteins/metabolism
MH  - Mice
MH  - Molecular Chaperones
MH  - Neoplasm Proteins/metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurofilament Proteins/*metabolism
MH  - Phospholipase C beta/metabolism
EDAT- 2010/02/04 06:00
MHDA- 2012/01/14 06:00
CRDT- 2010/02/04 06:00
PHST- 2010/02/04 06:00 [entrez]
PHST- 2010/02/04 06:00 [pubmed]
PHST- 2012/01/14 06:00 [medline]
AID - 10.1007/s12311-010-0156-y [doi]
PST - ppublish
SO  - Cerebellum. 2011 Sep;10(3):409-21. doi: 10.1007/s12311-010-0156-y.