PMID- 20128007
OWN - NLM
STAT- MEDLINE
DCOM- 20100817
LR  - 20231213
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 94
IP  - 1
DP  - 2010 Jul
TI  - The combination of micron and nanotopography by H(2)SO(4)/H(2)O(2) treatment and 
      its effects on osteoblast-specific gene expression of hMSCs.
PG  - 169-79
LID - 10.1002/jbm.a.32701 [doi]
AB  - H(2)SO(4)/H(2)O(2) treatment of titanium implants imparts nanofeatures to the 
      surface and alters the osteoblast response. The aim of this study was to evaluate 
      the effect of H(2)SO(4)/H(2)O(2) treatment of commercially pure Titanium (cpTi) 
      surfaces on gene expression of human mesenchymal stem cells (hMSCs) 
      differentiated into osteoblasts. Commercially pure grade IV titanium disks (20.0 
      mm x 1.0 mm) were polished or polished and subsequently treated by grit blasting 
      or grit-blasting/acid etching with an H(2)SO(4)/H(2)O(2) solution. The surfaces 
      were divided into three groups: smooth (S), grit-blasted (Gb), and 
      nanostructured: grit-blasted/acid etched (Nano). Surfaces were examined by 
      scanning electron microscopy and atomic force microscopy. HMSCs were grown on the 
      disks. The data points analyzed were at 3, 7, 14, and 28 days. Real-time PCR was 
      used to measure the mRNA levels of ALP, BSP, Runx2, OCN, OPN, and OSX. The 
      housekeeping gene GAPDH was used as a control. Descriptive statistics were 
      calculated using Microsoft Excel. T-test was performed for comparison of mRNA 
      levels when compared with S surfaces (p < 0.05). All osteoblast-specific genes 
      were regulated in surface-dependent patterns and most of them were upregulated on 
      the Nano surfaces. Runx2 and OSX mRNAs were more than threefold upregulated at 
      days 14 and 28 on Nano. Higher levels for ALP (38-fold), BSP (76-fold), and OCN 
      (3-fold) were also observed on the Nano surfaces. A grit-blasted surface imparted 
      with nanofeatures by H(2)SO(4)/H(2)O(2) treatment affected adherent cell 
      bone-specific gene expression. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater 
      Res, 2010.
FAU - Mendonca, Gustavo
AU  - Mendonca G
AD  - Universidade Catolica de Brasilia, Pos-Graduacao em Ciencias Genomicas e 
      Biotecnologia, SGAN Quadra 916, Modulo B, Av. W5 Norte 70.790-160-Asa Norte, 
      Brasilia/DF, Brazil. mendonca@ucb.br
FAU - Mendonca, Daniela B S
AU  - Mendonca DB
FAU - Aragao, Francisco J L
AU  - Aragao FJ
FAU - Cooper, Lyndon F
AU  - Cooper LF
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Biocompatible Materials)
RN  - 0 (Oxidants)
RN  - 0 (Sp7 Transcription Factor)
RN  - 0 (SP7 protein, human)
RN  - 0 (Sulfuric Acids)
RN  - 0 (Transcription Factors)
RN  - 104982-03-8 (Osteocalcin)
RN  - 106441-73-0 (Osteopontin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - D1JT611TNE (Titanium)
RN  - O40UQP6WCF (sulfuric acid)
SB  - IM
MH  - Biocompatible Materials/chemistry
MH  - Cell Adhesion
MH  - *Gene Expression
MH  - Humans
MH  - Hydrogen Peroxide/*chemistry
MH  - Materials Testing
MH  - Mesenchymal Stem Cells/cytology/*physiology
MH  - Microscopy, Atomic Force
MH  - Osseointegration
MH  - Osteoblasts/cytology/*physiology
MH  - Osteocalcin/genetics/metabolism
MH  - Osteopontin/genetics/metabolism
MH  - Oxidants/*chemistry
MH  - Prostheses and Implants
MH  - Sp7 Transcription Factor
MH  - Sulfuric Acids/*chemistry
MH  - Surface Properties
MH  - Titanium/chemistry
MH  - Transcription Factors/genetics/metabolism
EDAT- 2010/02/04 06:00
MHDA- 2010/08/18 06:00
CRDT- 2010/02/04 06:00
PHST- 2010/02/04 06:00 [entrez]
PHST- 2010/02/04 06:00 [pubmed]
PHST- 2010/08/18 06:00 [medline]
AID - 10.1002/jbm.a.32701 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2010 Jul;94(1):169-79. doi: 10.1002/jbm.a.32701.