PMID- 20128347
OWN - NLM
STAT- MEDLINE
DCOM- 20100426
LR  - 20100204
IS  - 0120-4157 (Print)
IS  - 0120-4157 (Linking)
VI  - 29
IP  - 2
DP  - 2009 Jun
TI  - [Clonal expansion and genomic characterization of the human T-cell lymphotropic 
      virus type I during the integration process in adult T-cell leukemia/lymphoma].
PG  - 218-31
AB  - INTRODUCTION: Although the integration of human T-cell lymphotropic virus type I 
      into the T-cells is not a random process, the mechanistic details are not 
      understood. OBJECTIVES: The characteristics of the flanking host chromatin were 
      evaluated at the integration sites in adult T-cell leukaemia/lymphoma (ATLL) 
      patients infected with the virus. MATERIALS AND METHODS: From seven leukemic 
      Colombian patients positive for the human T-cell lymphotropic virus type I 
      (HTLV-I), lymphocyte DNA samples were extracted and amplified by inverse 
      polymerase chain reaction (IPCR). Clonal expansion and human genome nucleotide 
      composition in an extension of 50 bp was determined. To establish the 
      characteristics of the human genome flanking provirus, 61 IPCR sequences from 
      Colombian and Japanese ATLL patients, were analyzed in silico to obtain insights 
      about the genomic structure, functions and nature of associated chromatin. 
      RESULTS: The clonal expansion of cell clones was predominantly oligoclonal. From 
      61 IPCR sequences, 155 alignments with homology higher than 95% (e-value < 0.05) 
      were screened. Seventy-five percent of those sequences corresponded to non coding 
      elements that include repetitive and non-repetitive DNA. Fifty percent of the 
      proviral integrations were associated with chromosomes of A and B groups. Viral 
      DNA integration tended to favor exons of genes that replicated early, controlled 
      the cell cycle, or were involved in signal transduction. CONCLUSIONS: The results 
      indicated that HTLV-I integration was preferentially directed towards genomic 
      environments with high C:G content, and toward genes that replicate early, 
      regulate cell cycle or involved with signal transduction.
FAU - Salcedo-Cifuentes, Mercedes
AU  - Salcedo-Cifuentes M
AD  - Laboratorio de Biologia Molecular y Patogenesis, Departamento de Ciencias 
      Fisiologicas, Facultad de Salud, Universidad del Valle, Cali, Colombia.
FAU - Cabrera, Jesus
AU  - Cabrera J
FAU - Cuesta-Astroz, Yesid
AU  - Cuesta-Astroz Y
FAU - Carrasca, Edwin
AU  - Carrasca E
FAU - Eizuru, Yoshito
AU  - Eizuru Y
FAU - Dominguez, Martha C
AU  - Dominguez MC
FAU - Sanchez, Adalberto
AU  - Sanchez A
FAU - Garcia-Vallejo, Felipe
AU  - Garcia-Vallejo F
LA  - spa
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
TT  - Expansion clonica y caracterizacion genomica del proceso de integracion del virus 
      linfotropico humano tipo I en la leucemia/linfoma de celulas T en adultos.
PL  - Colombia
TA  - Biomedica
JT  - Biomedica : revista del Instituto Nacional de Salud
JID - 8205605
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Base Composition
MH  - Cell Transformation, Viral/genetics
MH  - Child
MH  - Child, Preschool
MH  - Clone Cells/virology
MH  - DNA Replication/genetics
MH  - DNA, Neoplasm/genetics
MH  - DNA, Viral/genetics
MH  - Female
MH  - Genes, cdc
MH  - Genes, pX
MH  - *Genome, Viral
MH  - Human T-lymphotropic virus 1/genetics/isolation & purification/*physiology
MH  - Humans
MH  - Leukemia-Lymphoma, Adult T-Cell/*virology
MH  - Male
MH  - Middle Aged
MH  - Proviruses/*genetics/isolation & purification
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Signal Transduction/genetics
MH  - T-Lymphocytes/*virology
MH  - Virus Integration/*genetics
MH  - Young Adult
EDAT- 2010/02/05 06:00
MHDA- 2010/04/27 06:00
CRDT- 2010/02/05 06:00
PHST- 2010/02/05 06:00 [entrez]
PHST- 2010/02/05 06:00 [pubmed]
PHST- 2010/04/27 06:00 [medline]
AID - S0120-41572009000200007 [pii]
PST - ppublish
SO  - Biomedica. 2009 Jun;29(2):218-31.