PMID- 20129105
OWN - NLM
STAT- MEDLINE
DCOM- 20100420
LR  - 20131121
IS  - 1347-4421 (Electronic)
IS  - 1347-4421 (Linking)
VI  - 109
IP  - 2
DP  - 2010 Feb
TI  - Magnetic manipulation device for the optimization of cell processing conditions.
PG  - 182-8
LID - 10.1016/j.jbiosc.2009.07.006 [doi]
AB  - Variability in human cell phenotypes make it's advancements in optimized cell 
      processing necessary for personalized cell therapy. Here we propose a strategy of 
      palm-top sized device to assist physically manipulating cells for optimizing cell 
      preparations. For the design of such a device, we combined two conventional 
      approaches: multi-well plate formatting and magnetic cell handling using 
      magnetite cationic liposomes (MCLs). From our previous works, we showed the 
      labeling applications of MCL on adhesive cells for various tissue engineering 
      approaches. To feasibly transfer cells in multi-well plate, we here evaluated the 
      magnetic response of MCL-labeled suspension type cells. The cell handling 
      performance of Jurkat cells proved to be faster and more robust compared to MACS 
      (Magnetic Cell Sorting) bead methods. To further confirm our strategy, prototype 
      palm-top sized device "magnetic manipulation device (MMD)" was designed. In the 
      device, the actual cell transportation efficacy of Jurkat cells was satisfying. 
      Moreover, as a model of the most distributed clinical cell processing, primary 
      peripheral blood mononuclear cells (PBMCs) from different volunteers were 
      evaluated. By MMD, individual PBMCs indicated to have optimum Interleukin-2 
      (IL-2) concentrations for the expansion. Such huge differences of individual 
      cells indicated that MMD, our proposing efficient and self-contained support 
      tool, could assist the feasible and cost-effective optimization of cell 
      processing in clinical facilities.
CI  - Copyright (c) 2009 The Society for Biotechnology, Japan. Published by Elsevier 
      B.V. All rights reserved.
FAU - Ito, Hiroshi
AU  - Ito H
AD  - Department of Biotechnology, Graduate School of Engineering, Nagoya University, 
      Furo-cho, Chikusa-ku, Nagoya, Japan.
FAU - Kato, Ryuji
AU  - Kato R
FAU - Ino, Kosuke
AU  - Ino K
FAU - Honda, Hiroyuki
AU  - Honda H
LA  - eng
PT  - Journal Article
DEP - 20090812
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
RN  - 0 (Interleukin-2)
RN  - 0 (Liposomes)
RN  - XM0M87F357 (Ferrosoferric Oxide)
SB  - IM
MH  - Cell Separation/*instrumentation/*methods
MH  - Cell- and Tissue-Based Therapy
MH  - Cells, Cultured
MH  - Ferrosoferric Oxide
MH  - Humans
MH  - Interleukin-2/metabolism
MH  - Leukocytes, Mononuclear/cytology
MH  - Liposomes
MH  - *Magnetics
MH  - T-Lymphocytes/metabolism
EDAT- 2010/02/05 06:00
MHDA- 2010/04/21 06:00
CRDT- 2010/02/05 06:00
PHST- 2009/04/02 00:00 [received]
PHST- 2009/07/14 00:00 [revised]
PHST- 2009/07/15 00:00 [accepted]
PHST- 2010/02/05 06:00 [entrez]
PHST- 2010/02/05 06:00 [pubmed]
PHST- 2010/04/21 06:00 [medline]
AID - S1389-1723(09)00325-9 [pii]
AID - 10.1016/j.jbiosc.2009.07.006 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2010 Feb;109(2):182-8. doi: 10.1016/j.jbiosc.2009.07.006. Epub 
      2009 Aug 12.