PMID- 20129651
OWN - NLM
STAT- MEDLINE
DCOM- 20110624
LR  - 20161125
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 113
IP  - 3
DP  - 2011 May
TI  - Relationship between down-regulation of HIC1 and PTEN genes and dysfunction of 
      pancreatic islet cells in diabetic rats.
PG  - 340-8
LID - 10.1016/j.acthis.2010.01.005 [doi]
AB  - The aim of the study was to investigate the protein expression of hypermethylated 
      in cancer 1 (HIC1 ) and phosphatase and tensin homologue (PTEN) genes and to 
      study their mRNA expressions in normal and diabetic pancreatic islet cells in 
      rats in order to try and identify the functions of these genes in the development 
      and advancement of diabetes. We further aimed to analyze the expression of 
      mammalian target of rapamycin (mTOR), which is regulated by PTEN and to 
      investigate the possible mechanism of PTEN affecting the function of diabetic 
      islet cells. The expressions of HIC1, PTEN and mTOR genes were examined in the 
      pancreatic islets of 20 normal male Wistar rats and 47 diabetic male Wistar rats 
      by immunohistochemistry, Western blot, RT-PCR and real-time RT-PCR. Results 
      showed that expressions of HIC1 and PTEN in protein and mRNA levels were lower in 
      pancreatic islets of diabetic rats than in normal rats. Expressions of mTOR in 
      protein and mRNA levels were higher in pancreatic islets of diabetic rats than in 
      the normal rats. Marked apoptosis of pancreatic islet cells was observed in 29 
      cases (29/47, 61.7%) in diabetic rats, but not in the remaining 18 (18/47, 38.3%) 
      diabetic rats. The down-regulation of HIC1 and PTEN and up-regulation of mTOR in 
      protein and mRNA level are positively correlated with functional impairment of 
      islet cells in diabetic rats. From this study we conclude that HIC1, PTEN and 
      mTOR cannot be recognized as the key influencing factors promoting pancreatic 
      islet cells apoptosis of diabetic rats; however, lower expressions of HIC1 and 
      PTEN and higher expression of mTOR may affect the function of the pancreatic 
      islet cells in diabetic rats.
CI  - Copyright (c) 2010 Elsevier GmbH. All rights reserved.
FAU - Liu, Hao-ling
AU  - Liu HL
AD  - Department of Endocrinology, The First Clinical College of Harbin Medical 
      University, Harbin, PR China.
FAU - Yang, Hai-yan
AU  - Yang HY
FAU - Liu, Lian-xin
AU  - Liu LX
FAU - Chen, Yue
AU  - Chen Y
FAU - Kuang, Hong-yu
AU  - Kuang HY
FAU - Zhang, Hui-juan
AU  - Zhang HJ
FAU - Yin, Hui-qing
AU  - Yin HQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100202
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (HIC1 protein, human)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Diabetes Mellitus, Experimental/*physiopathology
MH  - *Down-Regulation
MH  - Immunohistochemistry
MH  - Islets of Langerhans/*pathology
MH  - Kruppel-Like Transcription Factors/genetics/*metabolism
MH  - Male
MH  - PTEN Phosphohydrolase/genetics/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2010/02/05 06:00
MHDA- 2011/06/28 06:00
CRDT- 2010/02/05 06:00
PHST- 2009/09/09 00:00 [received]
PHST- 2010/01/12 00:00 [revised]
PHST- 2010/01/13 00:00 [accepted]
PHST- 2010/02/05 06:00 [entrez]
PHST- 2010/02/05 06:00 [pubmed]
PHST- 2011/06/28 06:00 [medline]
AID - S0065-1281(10)00006-1 [pii]
AID - 10.1016/j.acthis.2010.01.005 [doi]
PST - ppublish
SO  - Acta Histochem. 2011 May;113(3):340-8. doi: 10.1016/j.acthis.2010.01.005. Epub 
      2010 Feb 2.