PMID- 20130128
OWN - NLM
STAT- MEDLINE
DCOM- 20100615
LR  - 20211020
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 17
IP  - 4
DP  - 2010 Apr
TI  - Comparative measurement of cell-mediated immune responses of swine to the M and N 
      proteins of porcine reproductive and respiratory syndrome virus.
PG  - 503-12
LID - 10.1128/CVI.00365-09 [doi]
AB  - The principal objectives of this study were to develop autologous 
      antigen-presenting cells (APCs) and to characterize the antigen-specific T-cell 
      responses to the M and N proteins of porcine reproductive and respiratory 
      syndrome virus (PRRSV) by using those APCs in outbred pigs. The orf6 and orf7 
      genes fused with porcine granulocyte-macrophage colony-stimulating factor 
      (GM-CSF) were cloned into the mammalian expression vector to generate two plasmid 
      DNAs, namely, pcDNA3.1-GM-CSF-PRRSV-M and pcDNA3.1-GM-CSF-PRRSV-N. Three of six 
      pigs in two groups were repeatedly immunized with either plasmid DNA construct, 
      and four pigs were used as controls. The recombinant M and N proteins fused with 
      the protein transduction domain (PTD) of the human immunodeficiency virus type 1 
      transactivator of transcription protein were employed to generate major 
      histocompatibility complex-matched autologous APCs from each pig. The levels of 
      T-cell proliferation and gamma interferon (IFN-gamma) synthesis were compared 
      between pigs immunized with the two plasmid DNAs after stimulation of the 
      peripheral blood mononuclear cells (PBMCs) of each pig with the autologous 
      antigen-presenting dendritic cells and PBMCs. Higher levels of T-cell 
      proliferation and IFN-gamma synthesis were identified in PBMCs isolated from the 
      pigs immunized with pcDNA3.1-GM-CSF-PRRSV-M than in those isolated from the pigs 
      immunized with pcDNA3.1-GM-CSF-PRRSV-N. By way of contrast, serum antibodies were 
      detected only in pigs immunized with pcDNA3.1-GM-CSF-PRRSV-N. However, no T-cell 
      response or antibody production was detected in the control pigs. These results 
      suggest that the M protein of PRRSV is a more potent T cell-stimulating antigen 
      than the N protein. Nevertheless, it should be emphasized that the N protein 
      substantially induces both cellular and humoral immune responses. The newly 
      developed protocol for generating self APCs may prove effective in further 
      efforts to characterize additional PRRSV proteins involved in the induction of 
      cell-mediated immunity.
FAU - Jeong, Hyun-Jeong
AU  - Jeong HJ
AD  - Department of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea.
FAU - Song, Young-Jo
AU  - Song YJ
FAU - Lee, Sang-Won
AU  - Lee SW
FAU - Lee, Joong-Bok
AU  - Lee JB
FAU - Park, Seung-Yong
AU  - Park SY
FAU - Song, Chang-Seon
AU  - Song CS
FAU - Ha, Gun-Woo
AU  - Ha GW
FAU - Oh, Jin-Sik
AU  - Oh JS
FAU - Oh, Youn-Kyoung
AU  - Oh YK
FAU - Choi, In-Soo
AU  - Choi IS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100203
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology
MH  - HIV-1/genetics
MH  - *Immunity, Cellular
MH  - Interferon-gamma/metabolism
MH  - Plasmids/administration & dosage
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - Swine
MH  - T-Lymphocytes/immunology
MH  - Vaccination/methods
MH  - Vaccines, DNA/administration & dosage
MH  - Viral Proteins/genetics/*immunology
PMC - PMC2849345
EDAT- 2010/02/05 06:00
MHDA- 2010/06/16 06:00
PMCR- 2010/10/01
CRDT- 2010/02/05 06:00
PHST- 2010/02/05 06:00 [entrez]
PHST- 2010/02/05 06:00 [pubmed]
PHST- 2010/06/16 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - CVI.00365-09 [pii]
AID - 0365-09 [pii]
AID - 10.1128/CVI.00365-09 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2010 Apr;17(4):503-12. doi: 10.1128/CVI.00365-09. Epub 2010 
      Feb 3.