PMID- 20133051
OWN - NLM
STAT- MEDLINE
DCOM- 20100621
LR  - 20220317
IS  - 1872-7980 (Electronic)
IS  - 0304-3835 (Linking)
VI  - 293
IP  - 1
DP  - 2010 Jul 1
TI  - Chrysin sensitizes tumor necrosis factor-alpha-induced apoptosis in human tumor 
      cells via suppression of nuclear factor-kappaB.
PG  - 109-16
LID - 10.1016/j.canlet.2010.01.002 [doi]
AB  - Chrysin (5,7-dihydroxyflavone) is a natural flavonoid commonly found in many 
      plants. The anti-cancer property of chrysin has been demonstrated although the 
      molecular mechanisms remain to be further elucidated. In the present study, we 
      found that, pretreatment with chrysin greatly sensitized various human cancer 
      cells to tumor necrosis factor-alpha (TNFalpha)-induced apoptosis. In the search 
      of the molecular mechanisms responsible for the sensitization effect of chrysin, 
      we discovered that such sensitization is closely associated with the inhibitory 
      effect of chrysin on TNFalpha-mediated nuclear transcription factor-kappaB 
      (NF-kappaB) activation. Pretreatment with chrysin inhibited TNFalpha-induced 
      degradation of Inhibitor of kappaB (IkappaB) protein and subsequent nuclear 
      translocation of p65. As a result, chrysin suppressed the expression of 
      NF-kappaB-targeted anti-apoptotic gene, c-FLIP-L. The role of c-FLIP-L was 
      further confirmed by its ectopic expression, which significantly protected cell 
      death induced by combined treatment with chrysin and TNFalpha. Data from this 
      study thus reveal a novel function of chrysin and enhance the value of chrysin as 
      an anti-cancer agent.
FAU - Li, Xin
AU  - Li X
AD  - Center for Disease Control and Prevention of Guangdong Province, 176 Xin Gang 
      Road, Guangzhou, Guangdong Province 510300, PR China.
FAU - Huang, Qing
AU  - Huang Q
FAU - Ong, Choon-Nam
AU  - Ong CN
FAU - Yang, Xing-Fen
AU  - Yang XF
FAU - Shen, Han-Ming
AU  - Shen HM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100204
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (CASP8 and FADD-Like Apoptosis Regulating Protein)
RN  - 0 (Flavonoids)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3CN01F5ZJ5 (chrysin)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - CASP8 and FADD-Like Apoptosis Regulating Protein/antagonists & 
      inhibitors/biosynthesis
MH  - Colorectal Neoplasms/*drug therapy
MH  - Down-Regulation/drug effects
MH  - Drug Synergism
MH  - Flavonoids/*pharmacology
MH  - HCT116 Cells
MH  - Hep G2 Cells
MH  - Humans
MH  - Immunoblotting
MH  - Liver Neoplasms/*drug therapy
MH  - NF-kappa B/antagonists & inhibitors/genetics/metabolism/*pharmacology
MH  - Nasopharyngeal Neoplasms/drug therapy
MH  - Neoplasms/*drug therapy/genetics/metabolism/pathology
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/*genetics/metabolism/pharmacology
EDAT- 2010/02/06 06:00
MHDA- 2010/06/22 06:00
CRDT- 2010/02/06 06:00
PHST- 2009/12/01 00:00 [received]
PHST- 2009/12/25 00:00 [revised]
PHST- 2010/01/11 00:00 [accepted]
PHST- 2010/02/06 06:00 [entrez]
PHST- 2010/02/06 06:00 [pubmed]
PHST- 2010/06/22 06:00 [medline]
AID - S0304-3835(10)00015-7 [pii]
AID - 10.1016/j.canlet.2010.01.002 [doi]
PST - ppublish
SO  - Cancer Lett. 2010 Jul 1;293(1):109-16. doi: 10.1016/j.canlet.2010.01.002. Epub 
      2010 Feb 4.