PMID- 20137148 OWN - NLM STAT- MEDLINE DCOM- 20111006 LR - 20161018 IS - 1009-2137 (Print) IS - 1009-2137 (Linking) VI - 18 IP - 1 DP - 2010 Feb TI - [Application of interphase FISH on cell smears in detection of hematological diseases]. PG - 204-7 AB - The study was aimed to investigate the application value of interphase fluorescence in situ hybridization (FISH) on cell smears in hematological diseases. Both interphase FISH on peripheral blood smears and bone marrow smears treated by methanol/acetic acid, and routine interphase FISH of bone marrow cells dropped on slides were done at the same time, in order to detect Ph chromosome by BCR/ABL dual color, dual fusion probe in 20 patients with chronic myelogenous leukemia or acute lymphoblastic leukemia which had been proven to display Ph chromosome positive. The results indicated that as compared with routine interphase FISH, the interphase FISH on cell smears could also offer reliable result. It is concluded that interphase FISH on cell smears is a kind of reliable and time-saving technique, which is also suitable for retrospective research and worthy to further apply in clinic. FAU - Sun, Wan-Ling AU - Sun WL AD - Department of Hematology, Xuanwu Hosptial, Capital Medical University, Beijing 100053, China. FAU - Liu, Cong-Yan AU - Liu CY FAU - Li, Hui AU - Li H FAU - He, Jing-Juan AU - He JJ FAU - Sun, Xue-Jing AU - Sun XJ FAU - Xu, Juan AU - Xu J LA - chi PT - English Abstract PT - Journal Article PL - China TA - Zhongguo Shi Yan Xue Ye Xue Za Zhi JT - Zhongguo shi yan xue ye xue za zhi JID - 101084424 SB - IM MH - Adult MH - Aged MH - Cytogenetic Analysis/*methods MH - Female MH - Hematologic Diseases/*diagnosis/genetics MH - Humans MH - In Situ Hybridization, Fluorescence/*methods MH - Interphase/genetics MH - Male MH - Middle Aged MH - Young Adult EDAT- 2010/02/09 06:00 MHDA- 2011/10/07 06:00 CRDT- 2010/02/09 06:00 PHST- 2010/02/09 06:00 [entrez] PHST- 2010/02/09 06:00 [pubmed] PHST- 2011/10/07 06:00 [medline] AID - 1009-2137(2010)01-0204-04 [pii] PST - ppublish SO - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Feb;18(1):204-7.