PMID- 20137424
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20181201
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 45
IP  - 11
DP  - 2009 Nov
TI  - [Experimental study of low molecular weight heparin drug delivery system for 
      prevention of posterior capsular opacification in rabbit eyes].
PG  - 1033-8
AB  - OBJECTIVE: To investigate the safety and efficacy of low-molecular-weight heparin 
      drug delivery system (LMWH DDS) for prevention of posterior capsular 
      opacification (PCO) in rabbit eyes. METHODS: (1) To prepare the LMWH DDS by 
      freeze-drying way with Polylactic-co-glycolic acid (PLGA) as the carrier, and 
      evaluate its release properties in vitro. (2) Fifty New Zealand albino rabbits 
      (50 eyes) undergoing phacoemulsification were equally divided into five groups: 
      receiving normal saline eye drops (group A), 3 different dose (1 mg, 0.5 mg and 
      0.25 mg) of LMWH DDS respectively implanted into the posterior chamber (group B, 
      C and D), and a carrier DDS implanted into the posterior chamber (group E). All 
      the 50 eyes were examined by slit-lamp microscopy. The low-molecular-weight 
      heparin levels in aqueous humor were measured, and the wet posterior capsules 
      were weighed. RESULTS: The LMWH DDS prepared with a freeze-dried way has high 
      encapsulation efficiency, and the equation of 49-day release curve fitting in 
      vitro were were similar to zero order. The fibrin exudation in group B, C and D 
      were lower than in Group A and E during the first postoperative day. There were 
      10, 2, 3, 9 and 10 eyes that developing PCO in the group A, B, C, D and E, 
      respectively. The mean wet-weight of the posterior capsule were (114.59 +/- 
      14.58) mg, (24.14 +/- 6.08) mg, (39.23 +/- 17.13) mg, (99.35 +/- 29.37) mg, 
      (115.29 +/- 19.87) mg respectively in 5 trial groups. There were stable and high 
      concentration of low molecular weight heparin in aqueous of group B and C during 
      the 4 weeks (> 20 mg/L), while a instable and lower concentrations in group D. 
      The result of optical microscopy and electron microscopy examination indicated 
      that fibroblast proliferation was quite active in groups A, D and E, but inactive 
      in group B and C. Neither infiltration of inflammatory cells at the cornea, iris, 
      trabecular meshwork and ciliary body nor retinal degeneration or necrosis was 
      found in any group at 12 weeks. There was no intraocular bleeding in all the five 
      groups in the following 12 weeks. CONCLUSIONS: The LMWH DDS prepared by 
      freeze-drying way with PLGA as the carrier has good slow-release and biological 
      tolerance. Implantation of LMWH DDS into the posterior chamber of experimental 
      animals can significantly reduce postoperative fibrin exudation, and can safe and 
      effective prevent the occurrence of PCO, and also there were some dose-effect 
      relationship.
FAU - Dai, Yun-Hai
AU  - Dai YH
AD  - State Key Laboratory Cultivation Base, Shandong Eye Institute, Qingdao 266071, 
      China.
FAU - Xie, Li-Xin
AU  - Xie LX
FAU - Wu, Xiang-Gen
AU  - Wu XG
FAU - Huang, Yu-Sen
AU  - Huang YS
FAU - Gong, Hua-Qing
AU  - Gong HQ
FAU - Yin, Hong-Mei
AU  - Yin HM
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Cataract/*prevention & control
MH  - *Drug Delivery Systems
MH  - Freeze Drying
MH  - Heparin, Low-Molecular-Weight/*administration & dosage/therapeutic use
MH  - Lactic Acid
MH  - Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Prospective Studies
MH  - Rabbits
EDAT- 2010/02/09 06:00
MHDA- 2011/11/16 06:00
CRDT- 2010/02/09 06:00
PHST- 2010/02/09 06:00 [entrez]
PHST- 2010/02/09 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 2009 Nov;45(11):1033-8.