PMID- 20138170
OWN - NLM
STAT- MEDLINE
DCOM- 20100525
LR  - 20161125
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 88
IP  - 3
DP  - 2010 Jun
TI  - Enhanced expression of lumican inhibited the attachment and growth of human 
      embryonic kidney 293 cells.
PG  - 363-70
LID - 10.1016/j.yexmp.2010.01.010 [doi]
AB  - Lumican is a member of a small leucine-rich proteoglycan (SLRP) family and it 
      regulates the assembly and diameter of collagen fibers in the extracellular 
      matrix of various tissues. Lumican expression was reported in various kinds of 
      tumor cells. Lumican inhibits the growth of melanoma cells, but the lumican in 
      pancreatic cancer correlated with an advanced stage and retroperitoneal and 
      duodenal invasion. In this study, we clarified whether the enhanced expression of 
      lumican contributes to cellular attachment, growth, colony formation, migration 
      and invasion. HEK 293 cell, stably transfected with lumican cDNA synthesized and 
      secreted a 50 kDa lumican protein at high levels in culture medium. The cells 
      showed a polygonal appearance with long projections and the degree of adhesion of 
      the cells to fibronectin was lower than that of empty vector transfected control 
      cells (mock cells). In contrast, the degree of adhesion of the cells to type I 
      collagen was not different from that of mock cells. The expression levels of 
      alpha5 integrin, the major integrin subunit for fibronectin, were lower in 
      lumican-transfected HEK cells than in mock cells. Furthermore, 
      lumican-transfected HEK cells showed reduced growth rates in vitro and did not 
      form colonies in soft agar. Phosphorylation of AKT, extracellular 
      signal-regulated kinase (ERK) 1/2 and mammalian target of rapamycin (mTOR) 
      decreased in the lumican-transfected HEK cells. Cell migration and invasion were 
      not altered in lumican-transfected HEK cells and mock cells. These findings 
      indicate that the 50kDa lumican protein plays important roles in the inhibition 
      of HEK cell attachment and growth, and it might inhibit the activation of 
      integrin pathways.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Ishiwata, Toshiyuki
AU  - Ishiwata T
AD  - Department of Pathology, Integrative Oncological Pathology, Nippon Medical 
      School, Tokyo, Japan. ishiwata@nms.ac.jp
FAU - Yamamoto, Tetsushi
AU  - Yamamoto T
FAU - Kawahara, Kiyoko
AU  - Kawahara K
FAU - Kawamoto, Yoko
AU  - Kawamoto Y
FAU - Matsuda, Yoko
AU  - Matsuda Y
FAU - Ishiwata, Shunji
AU  - Ishiwata S
FAU - Naito, Zenya
AU  - Naito Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100204
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Chondroitin Sulfate Proteoglycans)
RN  - 0 (Collagen Type I)
RN  - 0 (DNA Primers)
RN  - 0 (Fibronectins)
RN  - 0 (Integrins)
RN  - 0 (LUM protein, human)
RN  - 0 (Lumican)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 9056-36-4 (Keratan Sulfate)
SB  - IM
MH  - Base Sequence
MH  - Cell Adhesion/genetics/physiology
MH  - Cell Line
MH  - Cell Movement/genetics/physiology
MH  - Cell Proliferation
MH  - Chondroitin Sulfate Proteoglycans/chemistry/*genetics/physiology
MH  - Collagen Type I/metabolism
MH  - DNA Primers/genetics
MH  - Fibronectins/metabolism
MH  - Gene Expression
MH  - Glycosylation
MH  - Humans
MH  - Integrins/metabolism
MH  - Keratan Sulfate/chemistry/*genetics/physiology
MH  - Kidney/cytology/embryology/metabolism
MH  - Lumican
MH  - MAP Kinase Signaling System
MH  - Molecular Weight
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Signal Transduction
MH  - Transfection
EDAT- 2010/02/09 06:00
MHDA- 2010/05/26 06:00
CRDT- 2010/02/09 06:00
PHST- 2009/05/29 00:00 [received]
PHST- 2010/01/04 00:00 [revised]
PHST- 2010/01/25 00:00 [accepted]
PHST- 2010/02/09 06:00 [entrez]
PHST- 2010/02/09 06:00 [pubmed]
PHST- 2010/05/26 06:00 [medline]
AID - S0014-4800(10)00026-2 [pii]
AID - 10.1016/j.yexmp.2010.01.010 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2010 Jun;88(3):363-70. doi: 10.1016/j.yexmp.2010.01.010. Epub 
      2010 Feb 4.