PMID- 20138626
OWN - NLM
STAT- MEDLINE
DCOM- 20100518
LR  - 20131121
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1217
IP  - 11
DP  - 2010 Mar 12
TI  - On-line combination of single-drop liquid-liquid-liquid microextraction with 
      capillary electrophoresis for sample cleanup and preconcentration: a simple and 
      efficient approach to determining trace analyte in real matrices.
PG  - 1856-61
LID - 10.1016/j.chroma.2010.01.013 [doi]
AB  - In order to improve the sensitivity of capillary electrophoresis (CE) and 
      overcome the deficiency of commercial CE instruments in handling complex matrices 
      directly, we proposed a novel technique which combined single-drop 
      liquid-liquid-liquid microextraction (SD-LLLME) with CE on-line. In this 
      technique, SD-LLLME was realized using a commercial CE instrument and, to further 
      concentrate the target analyte, large-volume sample stacking combined sweeping 
      without polarity switching was utilized. Even though without agitating the donor 
      phase in the extraction process, the model compound, adenine was enriched 
      550-fold in only 10 min. The enrichment factors were 760 and 1030 when the 
      extraction time was extended to 30 and 60 min, respectively. The relative 
      standard deviations (RSDs) of adenine were 5.24% and 2.29% for peak area and 
      migration time, respectively, which indicated that this method was much more 
      reproducible compared to the existing methods that combined sample-preparation 
      strategies with CE. In addition, this approach was selective while cleaning up 
      target analyte. These mentioned advantages allowed the developed method to be an 
      attractive approach to determining trace target compounds in complex real 
      samples.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Zhu, Zaifang
AU  - Zhu Z
AD  - National Key Laboratory of Applied Organic Chemistry, Lanzhou University, South 
      Tianshui Road #222, Gansu, Lanzhou, China.
FAU - Zhou, Ximin
AU  - Zhou X
FAU - Yan, Na
AU  - Yan N
FAU - Zhou, Lei
AU  - Zhou L
FAU - Chen, Xingguo
AU  - Chen X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100114
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Acetates)
RN  - 0 (Tea)
RN  - 76845O8NMZ (ethyl acetate)
RN  - JAC85A2161 (Adenine)
RN  - QTT17582CB (Hydrochloric Acid)
SB  - IM
MH  - Acetates/chemistry
MH  - Adenine/analysis/chemistry
MH  - Chemical Fractionation/*methods
MH  - Electrophoresis, Capillary/*methods
MH  - Hydrochloric Acid/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Osmolar Concentration
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tea/chemistry
EDAT- 2010/02/09 06:00
MHDA- 2010/05/19 06:00
CRDT- 2010/02/09 06:00
PHST- 2009/09/16 00:00 [received]
PHST- 2009/12/22 00:00 [revised]
PHST- 2010/01/07 00:00 [accepted]
PHST- 2010/02/09 06:00 [entrez]
PHST- 2010/02/09 06:00 [pubmed]
PHST- 2010/05/19 06:00 [medline]
AID - S0021-9673(10)00050-6 [pii]
AID - 10.1016/j.chroma.2010.01.013 [doi]
PST - ppublish
SO  - J Chromatogr A. 2010 Mar 12;1217(11):1856-61. doi: 10.1016/j.chroma.2010.01.013. 
      Epub 2010 Jan 14.