PMID- 20139910
OWN - NLM
STAT- MEDLINE
DCOM- 20100615
LR  - 20230210
IS  - 1530-0285 (Electronic)
IS  - 0893-3952 (Linking)
VI  - 23
IP  - 4
DP  - 2010 Apr
TI  - Amplification of 8q21 in breast cancer is independent of MYC and associated with 
      poor patient outcome.
PG  - 603-10
LID - 10.1038/modpathol.2010.5 [doi]
AB  - Copy number gains involving the long arm of chromosome 8, including high-level 
      amplifications at 8q21 and 8q24, have been frequently reported in breast cancer. 
      Although the role of the MYC gene as the driver of the 8q24 amplicon is well 
      established, the significance of the 8q21 amplicon is less clear. The breast 
      cancer cell line SK-BR-3 contains three separate 8q21 amplicons, the distal two 
      of which correspond to putative target genes TPD52 and WWP1. To understand the 
      effect of proximal 8q21 amplification on breast cancer phenotype and patient 
      prognosis, we analyzed 8q21 copy number changes using fluorescence in situ 
      hybridization (FISH) in a tissue microarray containing more than 2000 breast 
      cancers. Amplification at 8q21 was found in 3% of tumors, and was associated with 
      medullary type (P<0.03), high tumor grade (P<0.0001), high Ki67 labeling index 
      (P<0.05), amplification of MYC (P<0.0001), HER2, MDM2, and CCND1 (P<0.05 each), 
      as well as the total number of gene amplifications (P<0.0001). 8q21 copy number 
      gains were significantly related to unfavorable patient outcome in univariate 
      analysis. However, multivariate Cox regression analysis did not reveal an 
      independent prognostic value of 8q21 amplification. The position of our FISH 
      probe and data of a previously performed high-resolution CGH study in the breast 
      cancer cell line SK-BR-3 involve TCEB1 and TMEM70 as new possible candidate 
      oncogenes at 8q21 in breast cancer.
FAU - Choschzick, Matthias
AU  - Choschzick M
AD  - Institute of Pathology, University Medical Centre Hamburg-Eppendorf, Hamburg, 
      Germany. mchoschz@uke.uni-hamburg.de
FAU - Lassen, Paula
AU  - Lassen P
FAU - Lebeau, Annette
AU  - Lebeau A
FAU - Marx, Andreas Holger
AU  - Marx AH
FAU - Terracciano, Luigi
AU  - Terracciano L
FAU - Heilenkotter, Uwe
AU  - Heilenkotter U
FAU - Jaenicke, Fritz
AU  - Jaenicke F
FAU - Bokemeyer, Carsten
AU  - Bokemeyer C
FAU - Izbicki, Jakob
AU  - Izbicki J
FAU - Sauter, Guido
AU  - Sauter G
FAU - Simon, Ronald
AU  - Simon R
LA  - eng
PT  - Journal Article
DEP - 20100205
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Breast Neoplasms/*genetics/mortality/*pathology
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Drug Resistance, Neoplasm/genetics
MH  - Female
MH  - Gene Amplification
MH  - Gene Dosage
MH  - Genes, myc
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kaplan-Meier Estimate
MH  - Middle Aged
MH  - Prognosis
MH  - Tissue Array Analysis
MH  - Treatment Outcome
EDAT- 2010/02/09 06:00
MHDA- 2010/06/16 06:00
CRDT- 2010/02/09 06:00
PHST- 2010/02/09 06:00 [entrez]
PHST- 2010/02/09 06:00 [pubmed]
PHST- 2010/06/16 06:00 [medline]
AID - S0893-3952(22)02740-5 [pii]
AID - 10.1038/modpathol.2010.5 [doi]
PST - ppublish
SO  - Mod Pathol. 2010 Apr;23(4):603-10. doi: 10.1038/modpathol.2010.5. Epub 2010 Feb 
      5.