PMID- 20140028
OWN - NLM
STAT- MEDLINE
DCOM- 20100427
LR  - 20221207
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 53
IP  - 2
DP  - 2010 Feb
TI  - Comparison of the chromosome maps around a resistance hot spot on chromosome 5 of 
      potato and tomato using BAC-FISH painting.
PG  - 103-10
LID - 10.1139/g09-086 [doi]
AB  - Potato chromosome 5 harbours numerous genes for important qualitative and 
      quantitative traits, such as resistance to the root cyst nematode Globodera 
      pallida and the late blight fungus, Phytophthora infestans. The genes make up 
      part of a "hot spot" for resistances to various pathogens covering a genetic map 
      length of 3 cM between markers GP21 and GP179. We established the physical size 
      and position of this region on chromosome 5 in potato and tomato using 
      fluorescence in situ hybridization (FISH) on pachytene chromosomes. Five potato 
      bacterial artificial chromosome (BAC) clones with the genetically anchored 
      markers GP21, R1-contig (proximal end), CosA, GP179, and StPto were selected, 
      labeled with different fluorophores, and hybridized in a five-colour FISH 
      experiment. Our results showed the location of the BAC clones in the middle of 
      the long arm of chromosome 5 in both potato and tomato. Based on chromosome 
      measurements, we estimate the physical size of the GP21-GP179 interval at 0.85 Mb 
      and 1.2 Mb in potato and tomato, respectively. The GP21-GP179 interval is part of 
      a genome segment known to have inverted map positions between potato and tomato.
FAU - Achenbach, Ute C
AU  - Achenbach UC
AD  - Max Planck Institute for Plant Breeding Research, Carl von Linne Weg 10, Koln, 
      Germany.
FAU - Tang, Xiaomin
AU  - Tang X
FAU - Ballvora, Agim
AU  - Ballvora A
FAU - de Jong, Hans
AU  - de Jong H
FAU - Gebhardt, Christiane
AU  - Gebhardt C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Genetic Markers)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Chromosomes, Artificial, Bacterial
MH  - *Chromosomes, Plant
MH  - Fluorescent Dyes/metabolism
MH  - Genetic Markers
MH  - Immunity, Innate/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles/metabolism
MH  - Solanum lycopersicum/*genetics
MH  - Physical Chromosome Mapping
MH  - Quantitative Trait Loci
MH  - Solanum tuberosum/*genetics
EDAT- 2010/02/09 06:00
MHDA- 2010/04/28 06:00
CRDT- 2010/02/09 06:00
PHST- 2010/02/09 06:00 [entrez]
PHST- 2010/02/09 06:00 [pubmed]
PHST- 2010/04/28 06:00 [medline]
AID - g09-086 [pii]
AID - 10.1139/g09-086 [doi]
PST - ppublish
SO  - Genome. 2010 Feb;53(2):103-10. doi: 10.1139/g09-086.