PMID- 20140426
OWN - NLM
STAT- MEDLINE
DCOM- 20100629
LR  - 20160512
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 396
IP  - 7
DP  - 2010 Apr
TI  - 2-(5-Benzoacridine)ethyl-p-toluenesulfonate as sensitive reagent for the 
      determination of bile acids by HPLC with fluorescence detection and online 
      atmospheric chemical ionization-mass spectrometric identification.
PG  - 2657-66
LID - 10.1007/s00216-010-3467-4 [doi]
AB  - 2-(5-Benzoacridine)ethyl-p-toluenesulfonate (BAETS), a dual-sensitive probe, was 
      reacted with bile acids in the presence of K(2)CO(3) catalyst in dimethyl 
      sulfoxide (DMSO) solvent to give BAETS-bile acid derivatives. Derivatives 
      exhibited intense fluorescence (FL) with an excitation maximum at lambda(ex) 270 
      nm and an emission maximum at lambda(em) 510 nm. MS analysis using APCI-MS 
      indicated that derivatives had excellent APCI-MS ionizability with percentage 
      ionization delta values changing from 0 to 88.83% in aqueous acetonitrile and 
      from 0 to 89.15% in aqueous methanol. The collision induced dissociation spectra 
      of m/z [M + H](+) contained specific fragment ions at m/z [M + H-H(2)O](+), [M + 
      H-2H(2)O](+), [M + H-3H(2)O](+), 347.3, and 290.1. Repeatability was good for LC 
      separation of BAETS-bile acid derivatives with aqueous acetonitrile as mobile 
      phase. The relative standard deviations (RSDs) of retention time and peak area at 
      6.6 nmol mL(-1) levels with fluorescence detection (FL) were from 0.045 to 0.072% 
      and from 2.16 to 2.73%, respectively. Excellent linear responses were observed, 
      with regression coefficients >0.9995. The FL detection limits (S/N = 3) were in 
      the range of 18.0-36.1 fmol. The online APCI-MS detection limits are in the range 
      of 500-790 fmol (at a signal-to-noise ratio of 3).
FAU - You, Jinmao
AU  - You J
AD  - Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 
      810001, China. Jmyou6304@163.com
FAU - Fu, Yanyan
AU  - Fu Y
FAU - Sun, Zhiwei
AU  - Sun Z
FAU - Suo, Yourui
AU  - Suo Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100206
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (2-(5-benzoacridine)ethyl-p-toluenesulfonate)
RN  - 0 (Acridines)
RN  - 0 (Benzenesulfonates)
RN  - 0 (Bile Acids and Salts)
RN  - 0 (Indicators and Reagents)
SB  - IM
MH  - Acridines/*chemistry
MH  - Atmospheric Pressure
MH  - Benzenesulfonates/*chemistry
MH  - Bile Acids and Salts/*analysis/*chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Indicators and Reagents/chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Fluorescence/*methods
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2010/02/09 06:00
MHDA- 2010/06/30 06:00
CRDT- 2010/02/09 06:00
PHST- 2009/10/13 00:00 [received]
PHST- 2010/01/08 00:00 [accepted]
PHST- 2010/01/02 00:00 [revised]
PHST- 2010/02/09 06:00 [entrez]
PHST- 2010/02/09 06:00 [pubmed]
PHST- 2010/06/30 06:00 [medline]
AID - 10.1007/s00216-010-3467-4 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2010 Apr;396(7):2657-66. doi: 10.1007/s00216-010-3467-4. Epub 
      2010 Feb 6.