PMID- 20140671
OWN - NLM
STAT- MEDLINE
DCOM- 20110310
LR  - 20211020
IS  - 1432-5195 (Electronic)
IS  - 0341-2695 (Print)
IS  - 0341-2695 (Linking)
VI  - 34
IP  - 8
DP  - 2010 Dec
TI  - Enhanced bone formation in large segmental radial defects by combining 
      adipose-derived stem cells expressing bone morphogenetic protein 2 with 
      nHA/RHLC/PLA scaffold.
PG  - 1341-9
LID - 10.1007/s00264-009-0946-3 [doi]
AB  - In this study, rabbit adipose-derived stem cells (rASCs) were isolated, cultured 
      in vitro, and transfected with recombinant adenovirus vector containing human 
      bone morphogenetic protein 2 (Ad-hBMP2). These cells were combined with a 
      nano-hydroxyapatite/recombinant human-like collagen/poly(lactic acid) scaffold 
      (nHA/RHLC/PLA) to fabricate a new biocomposite (hBMP2/rASCs-nHA/RHLC/PLA, group 
      1) and cultured in osteogenic medium. Non-transfected rASCs mixed with 
      nHA/RHLC/PLA (rASCs-nHA/RHLC/PLA, group 2) and nHA/RHLC/PLA scaffold alone (group 
      3) served as controls. Scanning electron microscope (SEM) demonstrated 
      integration of rASCs with the nHA/RHLC/PLA scaffold. Quantitative real-time 
      RT-PCR analyses of collagen I, osteonectin, and osteopontin cDNA expression 
      indicated that the osteogenic potency of rASCs was enhanced by transfection with 
      Ad-hBMP2. After in vitro culture for seven days, three groups were implanted into 
      15-mm length critical-sized segmental radial defects in rabbits. After 12 weeks, 
      radiographic and histological analyses were performed. In group 1, the medullary 
      cavity was recanalised, bone was rebuilt and moulding was finished, the bone 
      contour had begun to remodel and scaffold was degraded completely. In contrast, 
      bone defects were not repaired in groups 2 or 3. Furthermore, the scaffold 
      degradation rate in group 1 was significantly higher than in groups 2 or 3. In 
      summary, after transduction with Ad-hBMP2, the osteogenesis of rASCs was 
      enhanced; a new biocomposite created with these cells induced repair of a 
      critical bone defect in vivo in a relatively short time.
FAU - Hao, Wei
AU  - Hao W
AD  - Department of Orthopaedics & Traumatology, Provincial Hospital affiliated to 
      Shandong University, Ji'nan, People's Republic of China.
FAU - Dong, Jinlei
AU  - Dong J
FAU - Jiang, Ming
AU  - Jiang M
FAU - Wu, Junwei
AU  - Wu J
FAU - Cui, Fuzhai
AU  - Cui F
FAU - Zhou, Dongsheng
AU  - Zhou D
LA  - eng
PT  - Journal Article
DEP - 20100207
PL  - Germany
TA  - Int Orthop
JT  - International orthopaedics
JID - 7705431
RN  - 0 (BMP2 protein, human)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Osteonectin)
RN  - 106441-73-0 (Osteopontin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adipocytes/cytology/transplantation
MH  - Adult Stem Cells/cytology/metabolism/*transplantation
MH  - Animals
MH  - Biocompatible Materials
MH  - Bone Morphogenetic Protein 2/*metabolism
MH  - Bone Regeneration/*genetics
MH  - Cells, Cultured
MH  - Collagen/genetics/metabolism
MH  - Gene Expression
MH  - Humans
MH  - Osteogenesis/genetics
MH  - Osteonectin/genetics/metabolism
MH  - Osteopontin/genetics/metabolism
MH  - Rabbits
MH  - Radius/injuries/*pathology
MH  - Stem Cell Transplantation/*methods
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds
MH  - Transfection
PMC - PMC2989079
EDAT- 2010/02/09 06:00
MHDA- 2011/03/11 06:00
PMCR- 2011/12/01
CRDT- 2010/02/09 06:00
PHST- 2009/11/01 00:00 [received]
PHST- 2009/12/20 00:00 [accepted]
PHST- 2009/12/14 00:00 [revised]
PHST- 2010/02/09 06:00 [entrez]
PHST- 2010/02/09 06:00 [pubmed]
PHST- 2011/03/11 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - 946 [pii]
AID - 10.1007/s00264-009-0946-3 [doi]
PST - ppublish
SO  - Int Orthop. 2010 Dec;34(8):1341-9. doi: 10.1007/s00264-009-0946-3. Epub 2010 Feb 
      7.