PMID- 20140699
OWN - NLM
STAT- MEDLINE
DCOM- 20100817
LR  - 20211020
IS  - 1573-4838 (Electronic)
IS  - 0957-4530 (Linking)
VI  - 21
IP  - 4
DP  - 2010 Apr
TI  - Biocompatibility of individually designed scaffolds with human periosteum for use 
      in tissue engineering.
PG  - 1255-62
LID - 10.1007/s10856-009-3878-y [doi]
AB  - The aim of this study was to evaluate and compare the biocompatibility of 
      computer-assisted designed (CAD) synthetic hydroxyapatite (HA) and 
      tricalciumphosphate (TCP) blocks and natural bovine hydroxyapatite blocks for 
      augmentations and endocultivation by supporting and promoting the proliferation 
      of human periosteal cells. Human periosteum cells were cultured using an 
      osteogenic medium consisting of Dulbecco's modified Eagle medium supplemented 
      with fetal calf serum, Penicillin, Streptomycin and ascorbic acid at 37 degrees C 
      with 5% CO(2). Three scaffolds were tested: 3D-printed HA, 3D-printed TCP and 
      bovine HA. Cell vitality was assessed by Fluorescein Diacetate (FDA) and 
      Propidium Iodide (PI) staining, biocompatibility with LDH, MTT, WST and BrdU 
      tests, and scanning electron microscopy. Data were analyzed with ANOVAs. RESULTS: 
      After 24 h all samples showed viable periosteal cells, mixed with some dead cells 
      for the bovine HA group and very few dead cells for the printed HA and TCP 
      groups. The biocompatibility tests revealed that proliferation on all scaffolds 
      after treatment with eluate was sometimes even higher than controls. Scanning 
      electron microscopy showed that periosteal cells formed layers covering the 
      surfaces of all scaffolds 7 days after seeding. CONCLUSION: It can be concluded 
      from our data that the tested materials are biocompatible for periosteal cells 
      and thus can be used as scaffolds to augment bone using tissue engineering 
      methods.
FAU - Becker, Stephan T
AU  - Becker ST
AD  - Department of Oral and Maxillofacial Surgery, Christian-Albrechts-University of 
      Kiel, Kiel, Germany. becker@mkg.uni-kiel.de
FAU - Douglas, Timothy
AU  - Douglas T
FAU - Acil, Yahya
AU  - Acil Y
FAU - Seitz, Hermann
AU  - Seitz H
FAU - Sivananthan, Sureshan
AU  - Sivananthan S
FAU - Wiltfang, Jorg
AU  - Wiltfang J
FAU - Warnke, Patrick H
AU  - Warnke PH
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100207
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Adhesion/drug effects
MH  - Cell Culture Techniques
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Durapatite/chemistry/pharmacology
MH  - Humans
MH  - *Materials Testing
MH  - Models, Biological
MH  - Osteogenesis/drug effects
MH  - Periosteum/cytology/*physiology
MH  - Prosthesis Design/methods
MH  - *Tissue Engineering/methods
MH  - *Tissue Scaffolds/adverse effects/chemistry
EDAT- 2010/02/09 06:00
MHDA- 2010/08/18 06:00
CRDT- 2010/02/09 06:00
PHST- 2009/05/27 00:00 [received]
PHST- 2009/09/17 00:00 [accepted]
PHST- 2010/02/09 06:00 [entrez]
PHST- 2010/02/09 06:00 [pubmed]
PHST- 2010/08/18 06:00 [medline]
AID - 10.1007/s10856-009-3878-y [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2010 Apr;21(4):1255-62. doi: 10.1007/s10856-009-3878-y. 
      Epub 2010 Feb 7.