PMID- 20140876
OWN - NLM
STAT- MEDLINE
DCOM- 20100521
LR  - 20191210
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 27
IP  - 1
DP  - 2010 Feb
TI  - [Chromosome study on chronic lymphocytic leukemia using CpG-oligodeoxynucleotide 
      as immunostimulant agent].
PG  - 86-91
LID - 10.3760/cma.j.issn.1003-9406.2010.01.019 [doi]
AB  - OBJECTIVE: To investigate whether CpG-oligodeoxynucleotide (CpG-ODN) can improve 
      the detection rate of the karyotypic abnormalities in chronic lymphocytic 
      leukemia (CLL). METHODS: The bone marrow (BM) or peripheral blood (PB) cells from 
      57 cases of CLL were collected and cultured with CpG-ODN DSP30+interleukin-2 
      (IL-2), phytohemagglutinin (PHA), pokeweed (PWM) or IL-2, respectively. Five days 
      later cells were harvested for chromosome preparation. Karyotypic analysis was 
      done using R banding technique. Panel fluorescence in situ hybridization (FISH) 
      was carried out on 19 cases of CLL with normal karyotypes using the following 
      probes: Cen12, D13S25, Rb1, ATM, p53, MYB and IgH. Genomic DNA from 21 cases of 
      them was extracted from BM or PB leukocytes. The immunoglobulin variable heavy 
      chain (IgVH) was amplified by polymerase chain reaction (PCR) and sequenced. CD38 
      and ZAP70 expressions in the leukemic cells were determined by flow cytometry 
      (FCM). RESULTS: The detection rate of karyotypic abnormalities in the 
      CpG-ODN+IL-2 group (43.85%) was obviously higher than that in the PHA (15.09%), 
      PWM (17.31%) and IL-2 (3.13%) groups (P<0.01). Fifty-two types of karyotypic 
      abnormalities were found. Among them, trisomy12 (+12) or +12 with other 
      abnormalities were the most common, while translocations were the most frequent 
      structural abnormalities including 3 unbalanced and 11 balanced translocations, 
      among them 7 had rearrangements involving 14q32. Thirteen cases showed one or 
      more abnormalities on FISH including trisomy 12 and p53 deletion each in one 
      case, IgH rearrangement and partial deletion each in one case, 13q14.3 deletion 
      in 11 cases of which 5 cases also had Rb1 deletion, 1 case had Rb1 partial 
      deletion. No case with ATM or MYB deletions was found. PCR detected IgVH 
      mutations in 10/21 cases. FCM showed 10/45 cases were CD38 positive, but 35 /45 
      were CD38 negative, 11/27 cases expressed ZAP70, but 16/27 did not. Among the 26 
      cases examined for CD38 and ZAP70 expressions simultaneously, 5 cases were 
      CD38+ZAP70+, 13 were CD38-ZAP70-, 6 were CD38-ZAP70+, and 2 were CD38+ZAP70-, 
      respectively. Statistic analysis showed a correlation between complex karyotype 
      and IgVH without mutation, but no association between karyotype and CD38 or ZAP70 
      expression was observed. CONCLUSION: CpG-ODN immunostimulation can obviously 
      raise the detection rate of abnormal karyotypes, especially translocations in 
      CLL. FISH is an important complement to conventional karyotypic analysis. The 
      combination of both methods can provide more comprehensive genetic information 
      for CLL.
FAU - Wu, Yafang
AU  - Wu Y
AD  - The First Affiliated Hospital, Soochow University, Jiangsu Institute of 
      Hematology, Suzhou, Jiangsu, 215006 P.R.China.
FAU - Xue, Yongquan
AU  - Xue Y
FAU - Chen, Suning
AU  - Chen S
FAU - Yao, Li
AU  - Yao L
FAU - Jiang, Hui
AU  - Jiang H
FAU - Zhang, Jun
AU  - Zhang J
FAU - Shen, Juan
AU  - Shen J
FAU - Pan, Jinlan
AU  - Pan J
FAU - Wang, Yong
AU  - Wang Y
FAU - Bai, Shuxiao
AU  - Bai S
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (IL2 protein, human)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Interleukin-2)
RN  - 0 (Oligodeoxyribonucleotides)
SB  - IM
MH  - Adjuvants, Immunologic/*genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow Cells/cytology/immunology
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Interleukin-2/genetics
MH  - Karyotyping/*methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/*genetics/immunology
MH  - Male
MH  - Middle Aged
MH  - Oligodeoxyribonucleotides/*genetics/immunology
MH  - Phytolacca americana/genetics
EDAT- 2010/02/09 06:00
MHDA- 2010/05/22 06:00
CRDT- 2010/02/09 06:00
PHST- 2010/02/09 06:00 [entrez]
PHST- 2010/02/09 06:00 [pubmed]
PHST- 2010/05/22 06:00 [medline]
AID - 940627019 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2010.01.019 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Feb;27(1):86-91. doi: 
      10.3760/cma.j.issn.1003-9406.2010.01.019.