PMID- 20141586
OWN - NLM
STAT- MEDLINE
DCOM- 20100830
LR  - 20211020
IS  - 1743-6109 (Electronic)
IS  - 1743-6095 (Print)
IS  - 1743-6095 (Linking)
VI  - 7
IP  - 4 Pt 1
DP  - 2010 Apr
TI  - The effect of intracavernous injection of adipose tissue-derived stem cells on 
      hyperlipidemia-associated erectile dysfunction in a rat model.
PG  - 1391-400
LID - 10.1111/j.1743-6109.2009.01697.x [doi]
AB  - INTRODUCTION: Hyperlipidemia has been associated with erectile dysfunction (ED) 
      via damage to the cavernous endothelium and nerves. Adipose tissue-derived stem 
      cells (ADSC) have been shown to differentiate into endothelial cells and secrete 
      vasculotrophic and neurotrophic factors. AIM: To assess whether ADSC have 
      therapeutic effects on hyperlipidemia-associated ED. METHODS: Twenty-eight male 
      rats were induced to develop hyperlipidemia with a high-fat diet (hyperlipidemic 
      rats, HR). Ten additional male rats were fed a normal diet to serve as controls 
      (normal rats, NR). Five months later, all rats were subjected to ADSC isolation 
      from paragonadal fat. The cells were cultured for 1 week, labeled with 
      5-ethynyl-2'-deoxyuridine (EdU), and then injected autologously into the corpus 
      cavernosum of 18 HR. The remaining 10 HR rats were injected with phosphate 
      buffered saline (PBS). At 2 and 14 days post-transplantation, four rats in the HR 
      + ADSC group were sacrificed for tracking of the transplanted cells. At 28 days 
      post-transplantation, all remaining rats were analyzed for serum biochemistry, 
      erectile function, and penile histology. MAIN OUTCOME MEASURES: Erectile function 
      was assessed by intracavernous pressure (ICP) measurement during 
      electrostimulation of the cavernous nerve. Cavernous nerves, endothelium, and 
      smooth muscle were assessed by immunohistochemistry. RESULTS: Serum total 
      cholesterol and low-density lipoprotein levels were significantly higher in HR 
      than in NR. High-density lipoprotein level was significantly lower in HR than in 
      NR. Mean ICP/mean arterial pressure ratio was significantly lower in HR + PBS 
      than in NR + PBS or HR + ADSC. Neuronal nitric oxide synthase (nNOS)-positive 
      nerve fibers and endothelial cells were fewer in HR + PBS than in HR + ADSC. 
      Smooth muscle content was significantly higher in both HR groups than in NR. 
      CONCLUSIONS: Hyperlipidemia is associated with abnormalities in both the nerves 
      and endothelium. Treatment with ADSC ameliorates these adverse effects and holds 
      promise as a potential new therapy for ED.
FAU - Huang, Yun-Ching
AU  - Huang YC
AD  - Knuppe Molecular Urology Laboratory, Department of Urology, University of 
      California, San Francisco, CA, USA.
FAU - Ning, Hongxiu
AU  - Ning H
FAU - Shindel, Alan W
AU  - Shindel AW
FAU - Fandel, Thomas M
AU  - Fandel TM
FAU - Lin, Guiting
AU  - Lin G
FAU - Harraz, Ahmed M
AU  - Harraz AM
FAU - Lue, Tom F
AU  - Lue TF
FAU - Lin, Ching-Shwun
AU  - Lin CS
LA  - eng
GR  - R01 DK045370/DK/NIDDK NIH HHS/United States
GR  - R37 DK045370/DK/NIDDK NIH HHS/United States
GR  - R37 DK045370-15/DK/NIDDK NIH HHS/United States
GR  - DK045370/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100205
PL  - Netherlands
TA  - J Sex Med
JT  - The journal of sexual medicine
JID - 101230693
RN  - 0 (Lipoproteins, LDL)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Blood Pressure
MH  - Blood Vessels/pathology/physiopathology
MH  - Cholesterol/blood
MH  - Diet, Atherogenic
MH  - *Disease Models, Animal
MH  - Humans
MH  - Hyperlipidemias/pathology/*physiopathology
MH  - Impotence, Vasculogenic/pathology/*physiopathology
MH  - Lipoproteins, LDL/blood
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Nerve Fibers/pathology/physiology
MH  - Nitric Oxide Synthase Type I/metabolism
MH  - Penile Erection/physiology
MH  - Penis/*blood supply/*innervation/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Young Adult
PMC - PMC3163604
MID - NIHMS317705
EDAT- 2010/02/10 06:00
MHDA- 2010/08/31 06:00
PMCR- 2011/08/29
CRDT- 2010/02/10 06:00
PHST- 2010/02/10 06:00 [entrez]
PHST- 2010/02/10 06:00 [pubmed]
PHST- 2010/08/31 06:00 [medline]
PHST- 2011/08/29 00:00 [pmc-release]
AID - S1743-6095(15)32981-7 [pii]
AID - 10.1111/j.1743-6109.2009.01697.x [doi]
PST - ppublish
SO  - J Sex Med. 2010 Apr;7(4 Pt 1):1391-400. doi: 10.1111/j.1743-6109.2009.01697.x. 
      Epub 2010 Feb 5.