PMID- 20143464
OWN - NLM
STAT- MEDLINE
DCOM- 20100504
LR  - 20211020
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 16
IP  - 7
DP  - 2010 Feb 21
TI  - MicroRNA155 is induced in activated CD4(+) T cells of TNBS-induced colitis in 
      mice.
PG  - 854-61
AB  - AIM: To investigate the expression of microRNA155 (miRNA155) in trinitrobenzene 
      sulphonic acid (TNBS)-induced colitis and the relationship between miRNA155 and 
      tumor necrosis factor (TNF) expressions. METHODS: In TNBS colitis mice, miRNA155 
      and TNF mRNA expressions were measured in colons and CD4(+) T cells of draining 
      lymph nodes (LNs). CD4(+) T cells were cultured in vitro with or without 
      anti-CD3/CD28 antibody, and the expressions of miRNA155 and TNF mRNA in cells and 
      TNF concentration in culture media were examined. RESULTS: miRNA155 and TNF mRNA 
      expressions in colons and in cells of LNs were significantly increased in TNBS 
      colitis compared with controls. In TNBS colitis, miRNA155 and TNF mRNA 
      expressions in CD4(+) T cells of LNs and TNF concentration in CD4(+) T cells 
      culture media increased compared with controls. When cultured with anti-CD3/CD28 
      antibody, miRNA155 and TNF mRNA expressions in CD4(+) T cells and TNF 
      concentration in the CD4(+) T cells culture media were significantly higher than 
      those cultured without anti-CD3/CD28 antibody. Following analysis using the 
      Pearson's correlation coefficient, miRNA155 expression had a significant positive 
      correlation with either TNF mRNA expression in CD4(+) T cells (r = 0.860, P < 
      0.05) or TNF concentration in CD4(+) T cells culture media (r = 0.892, P < 0.05). 
      CONCLUSION: miRNA155 is induced in colons and activated CD4(+) T cells in TNBS 
      colitis, and the levels of miRNA155 and TNF expressions have a significant 
      positive correlation.
FAU - Chen, Da-Fan
AU  - Chen DF
AD  - Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University 
      School of Medicine, 197 Ruijin Second Road, Shanghai 200025, China.
FAU - Gong, Bang-Dong
AU  - Gong BD
FAU - Xie, Qing
AU  - Xie Q
FAU - Ben, Qi-Wen
AU  - Ben QW
FAU - Liu, Jun
AU  - Liu J
FAU - Yuan, Yao-Zong
AU  - Yuan YZ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn155 microRNA, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/immunology/*metabolism
MH  - Cells, Cultured
MH  - Colitis/chemically induced/*genetics/immunology
MH  - Colon/immunology/*metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Lymph Nodes/immunology/*metabolism
MH  - Lymphocyte Activation/*genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Trinitrobenzenesulfonic Acid
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
MH  - Up-Regulation
PMC - PMC2825332
EDAT- 2010/02/10 06:00
MHDA- 2010/05/05 06:00
PMCR- 2010/02/21
CRDT- 2010/02/10 06:00
PHST- 2010/02/10 06:00 [entrez]
PHST- 2010/02/10 06:00 [pubmed]
PHST- 2010/05/05 06:00 [medline]
PHST- 2010/02/21 00:00 [pmc-release]
AID - 10.3748/wjg.v16.i7.854 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2010 Feb 21;16(7):854-61. doi: 10.3748/wjg.v16.i7.854.