PMID- 20147642
OWN - NLM
STAT- MEDLINE
DCOM- 20101008
LR  - 20211020
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 48
IP  - 7
DP  - 2010 Jul
TI  - Multicenter external quality assessment of molecular methods for detection of 
      human herpesvirus 6.
PG  - 2536-40
LID - 10.1128/JCM.01145-09 [doi]
AB  - The purpose of this study was to evaluate the performance of laboratories for the 
      detection and quantification of human herpesvirus 6 (HHV-6) by an external 
      quality assessment (EQA) evaluation. The HHV-6 EQA panel consisted of eight 
      samples containing various concentrations of HHV-6 type A (strain GS) or type B 
      (strain Z29), two samples containing other herpesviruses (i.e., human 
      cytomegalovirus [HCMV] and Epstein-Barr virus [EBV]), and two HHV-6-negative 
      samples. Panel samples were prepared in human plasma, heat inactivated, and 
      lyophilized. Panel distribution, data management, and analysis were coordinated 
      by Quality Control for Molecular Diagnostics (QCMD), Glasgow, United Kingdom. 
      Fifty-one laboratories participated and submitted 57 data sets. Eleven (19.3%) 
      data sets were generated using conventional in-house assays, 11 (19.3%) data sets 
      using commercial real-time PCR assays, and 35 (61.4%) data sets using in-house 
      real-time PCR assays. The presence of HHV-6 DNA at viral loads exceeding 6,000 
      copies/ml was detected by all participants, and over 80% of the participants 
      still reported correct qualitative results for the sample containing just over 
      200 copies/ml. The false-positivity rate was 1.8% for both the negative samples 
      and the samples containing HCMV or EBV DNA. The majority (23/33; 69.7%) of 
      quantitative data sets were generated using in-house real-time PCR assays. The 
      standard deviations of the geometric means of the samples ranged from 0.5 to 0.7 
      log(10). The results of this first international EQA demonstrate encouraging 
      analytical sensitivity for the detection of HHV-6-DNA in human plasma, although 
      we observed extensive interlaboratory variation of quantitative HHV-6 DNA 
      results. Standardization needs to be improved to allow further elucidation of the 
      clinical significance of HHV-6 loads.
FAU - de Pagter, P J
AU  - de Pagter PJ
AD  - University Medical Center Utrecht, Department of Virology, Utrecht, Netherlands. 
      p.j.depagter@umcutrecht.nl
FAU - Schuurman, R
AU  - Schuurman R
FAU - de Vos, N M
AU  - de Vos NM
FAU - Mackay, W
AU  - Mackay W
FAU - van Loon, A M
AU  - van Loon AM
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
DEP - 20100210
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
MH  - DNA, Viral/analysis/isolation & purification
MH  - False Positive Reactions
MH  - *Herpesvirus 6, Human/genetics/isolation & purification
MH  - Humans
MH  - Laboratories/standards
MH  - *Molecular Diagnostic Techniques/methods/standards
MH  - Polymerase Chain Reaction
MH  - Quality Control
MH  - Roseolovirus Infections/diagnosis
MH  - Viral Load/*methods
MH  - Virology/*methods
PMC - PMC2897485
EDAT- 2010/02/12 06:00
MHDA- 2010/10/12 06:00
PMCR- 2011/01/01
CRDT- 2010/02/12 06:00
PHST- 2010/02/12 06:00 [entrez]
PHST- 2010/02/12 06:00 [pubmed]
PHST- 2010/10/12 06:00 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - JCM.01145-09 [pii]
AID - 1145-09 [pii]
AID - 10.1128/JCM.01145-09 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2010 Jul;48(7):2536-40. doi: 10.1128/JCM.01145-09. Epub 2010 
      Feb 10.