PMID- 20148748 OWN - NLM STAT- MEDLINE DCOM- 20100430 LR - 20131121 IS - 1091-7691 (Electronic) IS - 0895-8378 (Linking) VI - 22 IP - 3 DP - 2010 Feb TI - Cardiopulmonary response to inhalation of biogenic secondary organic aerosol. PG - 253-65 LID - 10.3109/08958370903148114 [doi] AB - An irradiation chamber designed for reproducible generation of inhalation test atmospheres of secondary organic aerosol (SOA) was used to evaluate cardiopulmonary responses in rodents exposed to SOA derived from the oxidation of alpha-pinene. SOA atmospheres were produced with 10:1 ratios of alpha-pinene:nitrogen oxides (NO(x)) and 10:1:1 ratios of alpha-pinene:nitrogen oxides:sulfur dioxide (SO(2)). SOA atmospheres were produced to yield 200 microg m(-3) of particulate matter (PM). Exposures were conducted downstream of honeycomb denuders employed to remove the gas-phase precursors and reaction products. Nose-only exposures were conducted with both rats (pulmonary effects) and mice (pulmonary and cardiovascular effects). Composition of the atmospheres was optimized to ensure that the SOA generated resembled SOA observed in previous irradiation studies, and contained specific SOA compounds of interest (e.g., organosulfates) identified in ambient air. Pulmonary and cardiovascular toxicity were measured in two different rodent species. In situ chemiluminescence and thiobarbituric acid- reactive substances (TBARS) were used to evaluate oxidative reactions in the F344 rats. ApoE(-/-) mice were exposed for 7 days and measurements of TBARS and gene expression of heme oxygenase-1 (HO-1), endothelin-1 (ET-1), matrix metalloproteinase-9 (MMP-9) were made in aorta. Pulmonary inflammatory responses in both species were measured by bronchoalveolar lavage fluid (BALF) cell counts. No pulmonary inflammation was observed in either species. A mild response was observed in mouse aorta for the upregulation of HO-1 and MMP-9, but was not seen for ET-1. Overall, alpha-pinene-derived SOA, including SOA that included organosulfate compounds, revealed limited biological response after short-term inhalation exposures. FAU - McDonald, Jacob D AU - McDonald JD AD - Lovelace Respiratory Research Institute, Albuquerque, New Mexico 87108, USA. jmcdonal@lrri.org FAU - Doyle-Eisele, Melanie AU - Doyle-Eisele M FAU - Campen, Matthew J AU - Campen MJ FAU - Seagrave, JeanClare AU - Seagrave J FAU - Holmes, Tom AU - Holmes T FAU - Lund, Amie AU - Lund A FAU - Surratt, Jason D AU - Surratt JD FAU - Seinfeld, John H AU - Seinfeld JH FAU - Rohr, Annette C AU - Rohr AC FAU - Knipping, Eladio M AU - Knipping EM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Inhal Toxicol JT - Inhalation toxicology JID - 8910739 RN - 0 (Aerosols) RN - 0 (Air Pollutants) RN - 0 (Apolipoproteins E) RN - 0 (Organic Chemicals) RN - 0 (Particulate Matter) RN - 0 (Thiobarbituric Acid Reactive Substances) RN - 0UZA3422Q4 (Sulfur Dioxide) SB - IM MH - *Aerosols MH - Air Pollutants/toxicity MH - Air Pollution/prevention & control MH - Animals MH - Apolipoproteins E/genetics MH - Atmosphere Exposure Chambers MH - Environmental Monitoring MH - Heart/*drug effects MH - Lung/*drug effects MH - Male MH - Mice MH - Mice, Knockout MH - Organic Chemicals/*toxicity MH - Oxidative Stress/drug effects MH - Particulate Matter/toxicity MH - Pneumonia/chemically induced/pathology MH - Rats MH - Rats, Inbred F344 MH - Rats, Sprague-Dawley MH - Sulfur Dioxide/administration & dosage/toxicity MH - Thiobarbituric Acid Reactive Substances/metabolism EDAT- 2010/02/13 06:00 MHDA- 2010/05/01 06:00 CRDT- 2010/02/13 06:00 PHST- 2010/02/13 06:00 [entrez] PHST- 2010/02/13 06:00 [pubmed] PHST- 2010/05/01 06:00 [medline] AID - 10.3109/08958370903148114 [doi] PST - ppublish SO - Inhal Toxicol. 2010 Feb;22(3):253-65. doi: 10.3109/08958370903148114.