PMID- 20149930 OWN - NLM STAT- MEDLINE DCOM- 20100803 LR - 20100505 IS - 1873-2534 (Electronic) IS - 0165-2427 (Linking) VI - 135 IP - 3-4 DP - 2010 Jun 15 TI - Characterization of anti-channel catfish IgL sigma monoclonal antibodies. PG - 325-8 LID - 10.1016/j.vetimm.2010.01.004 [doi] AB - This study characterizes three monoclonal antibodies (mAbs) developed against the constant (C) region of the immunoglobulin light (IgL) sigma chain isotype of the channel catfish, Ictalurus punctatus. Microsphere bead assays and Western blot analyses utilizing different recombinant (r) proteins show that these anti-catfish IgL sigma chain mAbs each specifically recognize the denatured form of IgL sigma. Importantly, Western blotting of catfish sera using the anti-IgL sigma mAbs also identified an IgL chain-sized immunoreactive band(s) of approximately 27kDa. It is anticipated that these mAbs in combination with the already existing anti-catfish Ig heavy (H) and IgL chain mAbs will be useful in future studies examining the functional roles of the different catfish IgL isotypes. CI - Copyright 2010 Elsevier B.V. All rights reserved. FAU - Edholm, Eva-Stina AU - Edholm ES AD - Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA. FAU - Hudgens, Edward D AU - Hudgens ED FAU - Tompkins, Dannielle AU - Tompkins D FAU - Sahoo, Manoranjan AU - Sahoo M FAU - Burkhalter, Bailey AU - Burkhalter B FAU - Miller, Norman W AU - Miller NW FAU - Bengten, Eva AU - Bengten E FAU - Wilson, Melanie AU - Wilson M LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20100125 PL - Netherlands TA - Vet Immunol Immunopathol JT - Veterinary immunology and immunopathology JID - 8002006 RN - 0 (Antibodies, Monoclonal) RN - 0 (DNA Primers) RN - 0 (Immunoglobulin Constant Regions) RN - 0 (Immunoglobulin Isotypes) RN - 0 (Immunoglobulin Light Chains) RN - 0 (Recombinant Proteins) SB - IM MH - Animals MH - Antibodies, Monoclonal/*immunology MH - Antibody Specificity MH - Base Sequence MH - DNA Primers/genetics MH - Ictaluridae/genetics/*immunology MH - Immunoglobulin Constant Regions/chemistry/genetics/immunology MH - Immunoglobulin Isotypes/chemistry/genetics/immunology MH - Immunoglobulin Light Chains/chemistry/genetics/*immunology MH - Molecular Weight MH - Protein Denaturation MH - Recombinant Proteins/chemistry/genetics/immunology EDAT- 2010/02/13 06:00 MHDA- 2010/08/04 06:00 CRDT- 2010/02/13 06:00 PHST- 2009/11/05 00:00 [received] PHST- 2010/01/12 00:00 [revised] PHST- 2010/01/20 00:00 [accepted] PHST- 2010/02/13 06:00 [entrez] PHST- 2010/02/13 06:00 [pubmed] PHST- 2010/08/04 06:00 [medline] AID - S0165-2427(10)00028-0 [pii] AID - 10.1016/j.vetimm.2010.01.004 [doi] PST - ppublish SO - Vet Immunol Immunopathol. 2010 Jun 15;135(3-4):325-8. doi: 10.1016/j.vetimm.2010.01.004. Epub 2010 Jan 25.