PMID- 20150565
OWN - NLM
STAT- MEDLINE
DCOM- 20100812
LR  - 20220408
IS  - 1522-1601 (Electronic)
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 108
IP  - 5
DP  - 2010 May
TI  - Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein 
      synthesis in older men.
PG  - 1199-209
LID - 10.1152/japplphysiol.01266.2009 [doi]
AB  - The loss of skeletal muscle mass during aging, sarcopenia, increases the risk for 
      falls and dependence. Resistance exercise (RE) is an effective rehabilitation 
      technique that can improve muscle mass and strength; however, older individuals 
      are resistant to the stimulation of muscle protein synthesis (MPS) with 
      traditional high-intensity RE. Recently, a novel rehabilitation exercise method, 
      low-intensity RE, combined with blood flow restriction (BFR), has been shown to 
      stimulate mammalian target of rapamycin complex 1 (mTORC1) signaling and MPS in 
      young men. We hypothesized that low-intensity RE with BFR would be able to 
      activate mTORC1 signaling and stimulate MPS in older men. We measured MPS and 
      mTORC1-associated signaling proteins in seven older men (age 70+/-2 yr) before 
      and after exercise. Subjects were studied identically on two occasions: during 
      BFR exercise [bilateral leg extension exercise at 20% of 1-repetition maximum 
      (1-RM) with pressure cuff placed proximally on both thighs and inflated at 200 
      mmHg] and during exercise without the pressure cuff (Ctrl). MPS and 
      phosphorylation of signaling proteins were determined on successive muscle 
      biopsies by stable isotopic techniques and immunoblotting, respectively. MPS 
      increased 56% from baseline after BFR exercise (P<0.05), while no change was 
      observed in the Ctrl group (P>0.05). Downstream of mTORC1, ribosomal S6 kinase 1 
      (S6K1) phosphorylation and ribosomal protein S6 (rpS6) phosphorylation increased 
      only in the BFR group after exercise (P<0.05). We conclude that low-intensity RE 
      in combination with BFR enhances mTORC1 signaling and MPS in older men. BFR 
      exercise is a novel intervention that may enhance muscle rehabilitation to 
      counteract sarcopenia.
FAU - Fry, Christopher S
AU  - Fry CS
AD  - University of Texas Medical Branch, Sealy Center on Aging, Department of Physical 
      Therapy, Division of Rehabilitation Sciences, 301 Univ. Blvd., Galveston, TX 
      77555-1144, USA.
FAU - Glynn, Erin L
AU  - Glynn EL
FAU - Drummond, Micah J
AU  - Drummond MJ
FAU - Timmerman, Kyle L
AU  - Timmerman KL
FAU - Fujita, Satoshi
AU  - Fujita S
FAU - Abe, Takashi
AU  - Abe T
FAU - Dhanani, Shaheen
AU  - Dhanani S
FAU - Volpi, Elena
AU  - Volpi E
FAU - Rasmussen, Blake B
AU  - Rasmussen BB
LA  - eng
GR  - AR-049877/AR/NIAMS NIH HHS/United States
GR  - 1UL1RR029876-01/RR/NCRR NIH HHS/United States
GR  - T32-HD-07539/HD/NICHD NIH HHS/United States
GR  - R01 AR049877/AR/NIAMS NIH HHS/United States
GR  - P30-AG-024832/AG/NIA NIH HHS/United States
GR  - P30 AG024832/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100211
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Biomarkers)
RN  - 0 (Fibrin Fibrinogen Degradation Products)
RN  - 0 (Hormones)
RN  - 0 (Insulin)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Muscle Proteins)
RN  - 0 (Proteins)
RN  - 0 (Ribosomal Protein S6)
RN  - 0 (Transcription Factors)
RN  - 0 (fibrin fragment D)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (ribosomal protein S6 kinase, 70kD, polypeptide 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Age Factors
MH  - Aged
MH  - Biomarkers/blood
MH  - Biopsy
MH  - Blotting, Western
MH  - Fibrin Fibrinogen Degradation Products/metabolism
MH  - Hormones/blood
MH  - Humans
MH  - Insulin/metabolism
MH  - Male
MH  - Mechanistic Target of Rapamycin Complex 1
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Multiprotein Complexes
MH  - *Muscle Contraction
MH  - Muscle Proteins/*biosynthesis/genetics
MH  - Organ Size
MH  - Oxygen/blood
MH  - Phosphorylation
MH  - Proteins
MH  - Quadriceps Muscle/anatomy & histology/*blood supply/*metabolism
MH  - Recovery of Function
MH  - Regional Blood Flow
MH  - *Resistance Training
MH  - Ribosomal Protein S6/metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Sex Factors
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases
MH  - Thrombosis/blood
MH  - Time Factors
MH  - Transcription Factors/*metabolism
PMC - PMC2867530
EDAT- 2010/02/13 06:00
MHDA- 2010/08/13 06:00
PMCR- 2011/05/01
CRDT- 2010/02/13 06:00
PHST- 2010/02/13 06:00 [entrez]
PHST- 2010/02/13 06:00 [pubmed]
PHST- 2010/08/13 06:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - 01266.2009 [pii]
AID - JAPPL-01266-2009 [pii]
AID - 10.1152/japplphysiol.01266.2009 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2010 May;108(5):1199-209. doi: 
      10.1152/japplphysiol.01266.2009. Epub 2010 Feb 11.