PMID- 20151325
OWN - NLM
STAT- MEDLINE
DCOM- 20111021
LR  - 20211203
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 38
IP  - 5
DP  - 2011 Jun
TI  - Leucine promotes leptin receptor expression in mouse C2C12 myotubes through the 
      mTOR pathway.
PG  - 3201-6
LID - 10.1007/s11033-010-9992-6 [doi]
AB  - Leptin plays a critical role in regulating muscle protein metabolism by binding 
      with leptin receptors in a 1:1 stoichiometry. However, the role for leucine in 
      the regulation of leptin receptor expression in muscle has not been investigated. 
      The present study was conducted to test the hypothesis that leucine regulates 
      leptin receptor levels in C2C12 myotubes. Cells were cultured in the presence of 
      DMEM/F12 medium containing supplemental 0 or 5 mM L: -leucine. Leptin receptor 
      expression by C2C12 myotubes peaked at 2 h post-supplementation. Additionally, 
      leucine stimulated leptin receptor expression at both mRNA and protein levels in 
      a dose-dependent manner. Furthermore, leucine enhanced the phosphorylation of 
      mammalian target of rapamycin (mTOR). Addition of rapamycin (an inhibitor of 
      mTOR) to culture medium completely suppressed leucine-induced activation of mTOR 
      and inhibited leucine-stimulated leptin receptor production. These results 
      indicate that leucine affects leptin receptor expression in muscle cells via the 
      mTOR signaling pathway.
FAU - Mao, Xiangbing
AU  - Mao X
AD  - State Key Laboratory on Animal Nutrition, China Agricultural University, No. 2. 
      Yuanmingyuan West Road, 100193 Beijing, China.
FAU - Zeng, Xiangfang
AU  - Zeng X
FAU - Wang, Junjun
AU  - Wang J
FAU - Qiao, Shiyan
AU  - Qiao S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100212
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Leptin)
RN  - 0 (Receptors, Leptin)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - GMW67QNF9C (Leucine)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Antibiotics, Antineoplastic/pharmacology
MH  - Cell Line
MH  - Leptin/metabolism
MH  - Leucine/*metabolism
MH  - Mice
MH  - Muscle Fibers, Skeletal/cytology/drug effects/*physiology
MH  - Receptors, Leptin/genetics/*metabolism
MH  - Signal Transduction/*physiology
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
EDAT- 2010/02/13 06:00
MHDA- 2011/10/22 06:00
CRDT- 2010/02/13 06:00
PHST- 2009/10/15 00:00 [received]
PHST- 2010/02/03 00:00 [accepted]
PHST- 2010/02/13 06:00 [entrez]
PHST- 2010/02/13 06:00 [pubmed]
PHST- 2011/10/22 06:00 [medline]
AID - 10.1007/s11033-010-9992-6 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2011 Jun;38(5):3201-6. doi: 10.1007/s11033-010-9992-6. Epub 2010 
      Feb 12.