PMID- 20153036
OWN - NLM
STAT- MEDLINE
DCOM- 20100407
LR  - 20211028
IS  - 1097-6825 (Electronic)
IS  - 0091-6749 (Linking)
VI  - 125
IP  - 3
DP  - 2010 Mar
TI  - Induction of anergic allergen-specific suppressor T cells using tolerogenic 
      dendritic cells derived from children with allergies to house dust mites.
PG  - 727-36
LID - 10.1016/j.jaci.2009.12.004 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) regulate the immune response to allergens in 
      the lung; they induce either effector or regulatory T cells, which promote or 
      suppress, respectively, the development of allergy. IL-10 is a potent 
      immunosuppressive cytokine that induces type 1 regulatory (Tr1) T cells. 
      OBJECTIVE: To generate allergen-specific Tr1 cells in vitro from children with 
      allergy. METHODS: Monocyte-derived DCs from children with allergy to house dust 
      mites (HDM) were generated by incubating the cells with IL-10 and pulsing them 
      with Der p 2, a major HDM allergen, or by pulsing them with Der p 2 and 
      incubating them with IL-10 during their last 2 days of differentiation. RESULTS: 
      Der p 2-specific T-cell proliferation and T(H)2 cytokine production were 
      significantly reduced when T cells from patients with allergy to HDM were 
      activated with autologous Der p 2-pulsed DCs that had been differentiated or 
      incubated with IL-10. T-cell lines generated with Der p 2-pulsed DCs that were 
      differentiated with IL-10 were hyporesponsive to reactivation with Der p 2 and 
      able to suppress Der p 2-specific T(H)2 effector cells. CONCLUSION: Dendritic 
      cells differentiated in the presence of IL-10 and pulsed with allergen gave rise 
      to a population of tolerogenic DCs that induced allergen-specific Tr1 cells. This 
      finding represents an important step forward to the prospective clinical 
      application of tolerogenic DCs to modulate allergen-specific T-cell responses.
FAU - Pacciani, Valentina
AU  - Pacciani V
AD  - Department of Pediatric Medicine, Immuno-Infectivology Unit, Bambino Gesu' 
      Children's Hospital, Rome, Italy.
FAU - Gregori, Silvia
AU  - Gregori S
FAU - Chini, Loredana
AU  - Chini L
FAU - Corrente, Stefania
AU  - Corrente S
FAU - Chianca, Marco
AU  - Chianca M
FAU - Moschese, Viviana
AU  - Moschese V
FAU - Rossi, Paolo
AU  - Rossi P
FAU - Roncarolo, Maria Grazia
AU  - Roncarolo MG
FAU - Angelini, Federica
AU  - Angelini F
LA  - eng
GR  - TGT06S01/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100211
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Cytokines)
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (Dermatophagoides pteronyssinus antigen p 1)
SB  - IM
MH  - Adolescent
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Arthropod Proteins
MH  - Cell Differentiation/*immunology
MH  - Cell Proliferation
MH  - Child
MH  - Child, Preschool
MH  - Clonal Anergy/immunology
MH  - Cysteine Endopeptidases
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/cytology/*immunology
MH  - Female
MH  - Humans
MH  - Hypersensitivity/immunology
MH  - Immune Tolerance/*immunology
MH  - Interleukin-10/immunology
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Pyroglyphidae/immunology
MH  - T-Lymphocytes, Regulatory/cytology/*immunology
EDAT- 2010/02/16 06:00
MHDA- 2010/04/08 06:00
CRDT- 2010/02/16 06:00
PHST- 2009/03/10 00:00 [received]
PHST- 2009/11/17 00:00 [revised]
PHST- 2009/12/02 00:00 [accepted]
PHST- 2010/02/16 06:00 [entrez]
PHST- 2010/02/16 06:00 [pubmed]
PHST- 2010/04/08 06:00 [medline]
AID - S0091-6749(09)01796-5 [pii]
AID - 10.1016/j.jaci.2009.12.004 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2010 Mar;125(3):727-36. doi: 10.1016/j.jaci.2009.12.004. 
      Epub 2010 Feb 11.