PMID- 20153851
OWN - NLM
STAT- MEDLINE
DCOM- 20100715
LR  - 20131121
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 55
IP  - 4
DP  - 2010 Apr
TI  - 17beta-Estradiol accentuates contractility of rat genioglossal muscle via 
      regulation of estrogen receptor alpha.
PG  - 309-17
LID - 10.1016/j.archoralbio.2010.02.002 [doi]
AB  - OBJECTIVE: This study in rat genioglossus muscle (GG) was designed to test the 
      hypothesis that the effects of estrogen are at least in part, meditated directly 
      by the estrogen receptors (ERs) of muscle. DESIGN: Eighty-eight-week-old female 
      Sprague-Dawley rats were randomly assigned to five groups: (1) normal animals 
      (Normal); (2) sham operation animals (Sham); (3) ovariectomized animals without 
      estrogen replacement (OVX); (4) ovariectomized animals with olive oil replacement 
      (OVX+O); (5) ovariectomized animals with 17beta-estradiol replacement (OVX+E2). 
      Six weeks later, GG was assessed in vivo for contractile properties and further 
      analysis for ERs expression was carried out including real-time quantitative 
      RT-PCR, immunohistochemistry and Western blotting. RESULTS: The maximal twitch 
      tension, 70%-decay time and fatigue index of GG decreased significantly in OVX 
      group when compared with Normal group (P<0.05, P<0.05, P<0.05). However, all the 
      three parameters reversed in OVX+E2 group especially fatigue index. Further 
      analysis showed a clear expression of ERalpha and ERbeta in rat GG. The 
      expression of both ERalpha protein and ERalpha mRNA was both significantly 
      decreased in OVX group (P<0.05) and recovered back to previous level after 
      receiving 17beta-estradiol replacement (P<0.05). But neither ERbeta protein nor 
      ERbeta mRNA was regulated by estrogen deprivation and replacement. CONCLUSION: 
      The results demonstrated that the contractility of GG was accentuated by 
      estrogen. Moreover, these effects were at least in part, meditated directly via 
      regulation of the expression of ERalpha. It might contribute to the protective 
      effects of estrogen on the patency of upper airway and the pathogenesis of 
      obstructive sleep apnea hypopnoea syndrome.
FAU - Hou, Yu-xia
AU  - Hou YX
AD  - Department of Orthodontics, School of Stomatology, Tongji University, 399 
      Yanchangzhong Road, Shanghai 200072, China.
FAU - Jia, Shan-shan
AU  - Jia SS
FAU - Liu, Yue-hua
AU  - Liu YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100213
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Estrogen Receptor alpha)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Blotting, Western
MH  - Estradiol/blood/*pharmacology
MH  - Estrogen Receptor alpha/*metabolism
MH  - Female
MH  - Immunoenzyme Techniques
MH  - Muscle Contraction/*drug effects
MH  - Ovariectomy
MH  - Pharyngeal Muscles/*drug effects/metabolism
MH  - Progesterone/blood
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2010/02/16 06:00
MHDA- 2010/07/16 06:00
CRDT- 2010/02/16 06:00
PHST- 2009/03/23 00:00 [received]
PHST- 2010/01/26 00:00 [revised]
PHST- 2010/02/01 00:00 [accepted]
PHST- 2010/02/16 06:00 [entrez]
PHST- 2010/02/16 06:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
AID - S0003-9969(10)00029-4 [pii]
AID - 10.1016/j.archoralbio.2010.02.002 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2010 Apr;55(4):309-17. doi: 10.1016/j.archoralbio.2010.02.002. 
      Epub 2010 Feb 13.