PMID- 20155312
OWN - NLM
STAT- MEDLINE
DCOM- 20100629
LR  - 20211020
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 40
IP  - 3
DP  - 2010 Jun
TI  - Genomic analysis of two Chinese strains of porcine reproductive and respiratory 
      syndrome viruses with different virulence.
PG  - 374-81
LID - 10.1007/s11262-010-0453-z [doi]
AB  - Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were 
      isolated, designated GDQJ and GDBY1. Experimental inoculation showed that GDBY1, 
      caused 100% morbidity and 67% mortality, while GDQJ, caused 100% morbidity but no 
      death. Full-length genomes were sequenced. Homologic and phylogenetic analyses 
      indicated that these two strains were closely related to Chinese highly 
      pathogenic PRRSV strains. Surprisingly, identical 30 amino acids (aa) deletion in 
      the NSP2-coding region, a presumed high virulence marker, was present in low 
      virulent strain GDQJ. Further comprehensive analysis of GDQJ genome in comparison 
      with Chinese highly pathogenic PRRSV strains revealed multiple genomic 
      variations, distributing in 5' UTR, NSP1b, NSP2, NSP3, NSP5, NSP7, NSP9, NSP10, 
      GP5, and N regions. Data present in this article confirm that the 30 aa deletion 
      in the NSP2-coding region alone is not a reliable genomic indicator for the high 
      virulence of PRRSV strains emerged in China. The genomic variations of GDQJ 
      strain provided the basis for further studies of virulence determinants for 
      PRRSVs.
FAU - Li, Yan
AU  - Li Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen 
      University, Guangzhou 510006, People's Republic of China.
FAU - Xue, Chunyi
AU  - Xue C
FAU - Wang, Lianxiang
AU  - Wang L
FAU - Chen, Xianxian
AU  - Chen X
FAU - Chen, Feng
AU  - Chen F
FAU - Cao, Yongchang
AU  - Cao Y
LA  - eng
SI  - GENBANK/GQ374441
SI  - GENBANK/GQ374442
PT  - Journal Article
DEP - 20100213
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - China
MH  - Female
MH  - Genome, Viral
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymorphism, Genetic
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification/*pathogenicity
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion
MH  - Sequence Homology
MH  - Survival Analysis
MH  - Swine
MH  - Viral Nonstructural Proteins/genetics
MH  - Virulence
MH  - Virulence Factors/genetics
EDAT- 2010/02/16 06:00
MHDA- 2010/06/30 06:00
CRDT- 2010/02/16 06:00
PHST- 2009/09/14 00:00 [received]
PHST- 2010/01/27 00:00 [accepted]
PHST- 2010/02/16 06:00 [entrez]
PHST- 2010/02/16 06:00 [pubmed]
PHST- 2010/06/30 06:00 [medline]
AID - 10.1007/s11262-010-0453-z [doi]
PST - ppublish
SO  - Virus Genes. 2010 Jun;40(3):374-81. doi: 10.1007/s11262-010-0453-z. Epub 2010 Feb 
      13.