PMID- 2015607
OWN - NLM
STAT- MEDLINE
DCOM- 19910517
LR  - 20151119
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 51
IP  - 9
DP  - 1991 May 1
TI  - Effect of transforming growth factor beta on cell death of cultured rat 
      hepatocytes.
PG  - 2478-85
AB  - We investigate mechanisms of regression of liver hyperplasia which occurs after 
      induction of growth by hepatomitogens and their subsequent withdrawal. We 
      hypothesized that transforming growth factor beta 1 (TGF-beta 1) might be 
      involved in the control of regression. Therefore we studied the effect of this 
      agent on DNA synthesis and death of hepatocytes cultured in vitro. Both the low 
      basal rate of DNA synthesis of untreated cells and its increase by epidermal 
      growth factor (10 ng/ml) were suppressed by TGF-beta 1 at concentrations higher 
      than 0.01-0.1 ng/ml. At the same range of concentrations of TGF-beta 1, the DNA 
      content of the cultures declined significantly and numerous dead cells could be 
      seen in the monolayer. Time course studies showed that TGF-beta 1 (1 ng/ml) 
      decreased DNA content in the cultures linearly to 41 +/- 7% of controls during a 
      period of 48 h. A similar decrease occurred with vital hepatocytes in hematoxylin 
      and eosin stained monolayers. These changes were accompanied by an extensive 
      release of lactate dehydrogenase which began at 20 h and was 70% of the total 
      lactate dehydrogenase content of the cultures at 40-48 h. Little formation of 
      guanidine hydrochloride resistant bodies and no fragmentation of DNA, indicators 
      of apoptotic cell death, were detected after TGF-beta 1 (1 ng/ml) treatment. Time 
      lapse cinematography revealed an active detachment of the cells from the 
      underlying collagen gel. These studies show that inhibition of DNA synthesis by 
      TGF-beta 1 is associated with enhanced cell death in cultured hepatocytes.
FAU - Oberhammer, F
AU  - Oberhammer F
AD  - Institut fur Tumorbiologie-Krebsforschung Universitat Wien, Vienna, Austria.
FAU - Bursch, W
AU  - Bursch W
FAU - Parzefall, W
AU  - Parzefall W
FAU - Breit, P
AU  - Breit P
FAU - Erber, E
AU  - Erber E
FAU - Stadler, M
AU  - Stadler M
FAU - Schulte-Hermann, R
AU  - Schulte-Hermann R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Biomarkers)
RN  - 0 (Transforming Growth Factor beta)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Cell Division
MH  - Cell Survival/*drug effects
MH  - Cells, Cultured
MH  - DNA/*biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Epidermal Growth Factor/pharmacology
MH  - Female
MH  - Liver/*cytology/metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Time Factors
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1991/05/01 00:00
MHDA- 1991/05/01 00:01
CRDT- 1991/05/01 00:00
PHST- 1991/05/01 00:00 [pubmed]
PHST- 1991/05/01 00:01 [medline]
PHST- 1991/05/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1991 May 1;51(9):2478-85.