PMID- 20156507
OWN - NLM
STAT- MEDLINE
DCOM- 20100709
LR  - 20100419
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 71
IP  - 5
DP  - 2010 May
TI  - New HLA class I epitopes defined by murine monoclonal antibodies.
PG  - 456-61
LID - 10.1016/j.humimm.2010.02.011 [doi]
AB  - This study defines 10 epitopes by murine monoclonal antibodies, of which seven 
      are new and three were previously defined by alloantibodies. Of particular 
      interest, three antibodies reacted with almost all Bw4-associated antigens except 
      that each was negative with one or two of the antigens. One was negative with 
      B13, one negative with A25, and another negative with B13 & A24 antigens. These 
      monoclonal antibodies exhibited reactivity contrary to Bw4 allosera, which 
      typically react with all Bw4-associated antigens. All monoclonal antibodies were 
      tested with a panel of 97 human leukocyte antigen (HLA) class I (A, B, and 
      Clocus) rHLA single antigens (SA) individually coupled to different microsphere 
      beads. Identifying HLA antigens sharing distinct epitopes can be helpful when 
      selecting patient-donor transplantation pairs, explaining antibodies against rare 
      specificities, or against non-donor-specific antigens (NDSA). This study adds 
      seven new HLA class I epitopes to 103 already defined epitopes and provides more 
      evidence that a monoclonal antibody and alloantibody can target the same epitope. 
      The fact that mAbs can target the same epitopes targeted by allosera, makes mAbs 
      useful in studies of cross-reactivity in HLA.
CI  - Copyright 2010 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - El-Awar, Nadim
AU  - El-Awar N
AD  - One Lambda Inc., Canoga Park, Los Angeles, California, USA. nelawar@onelambda.com
FAU - Terasaki, Paul I
AU  - Terasaki PI
FAU - Nguyen, Anh
AU  - Nguyen A
FAU - Lias, Mamie
AU  - Lias M
FAU - Conger, Nathan
AU  - Conger N
LA  - eng
PT  - Journal Article
DEP - 20100307
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Specificity/*immunology
MH  - Epitopes, B-Lymphocyte/chemistry/*immunology
MH  - Histocompatibility Antigens Class I/chemistry/*immunology
MH  - Humans
MH  - Mice
EDAT- 2010/02/17 06:00
MHDA- 2010/07/10 06:00
CRDT- 2010/02/17 06:00
PHST- 2009/12/16 00:00 [received]
PHST- 2010/02/05 00:00 [revised]
PHST- 2010/02/09 00:00 [accepted]
PHST- 2010/02/17 06:00 [entrez]
PHST- 2010/02/17 06:00 [pubmed]
PHST- 2010/07/10 06:00 [medline]
AID - S0198-8859(10)00054-6 [pii]
AID - 10.1016/j.humimm.2010.02.011 [doi]
PST - ppublish
SO  - Hum Immunol. 2010 May;71(5):456-61. doi: 10.1016/j.humimm.2010.02.011. Epub 2010 
      Mar 7.