PMID- 20156944
OWN - NLM
STAT- MEDLINE
DCOM- 20100622
LR  - 20100402
IS  - 1499-2752 (Electronic)
IS  - 0315-162X (Linking)
VI  - 37
IP  - 4
DP  - 2010 Apr
TI  - Thrombin-cleaved osteopontin is increased in urine of patients with rheumatoid 
      arthritis.
PG  - 704-10
LID - 10.3899/jrheum.090582 [doi]
AB  - OBJECTIVE: To measure concentrations of the thrombin-cleaved isoform of 
      osteopontin (OPN) in urine and plasma of patients with rheumatoid arthritis (RA), 
      and to assess whether levels of thrombin-cleaved OPN are associated with measures 
      of RA. METHODS: Subjects comprised 70 patients with RA, 20 patients with 
      osteoarthritis (OA), and 46 healthy controls. RA disease activity was evaluated 
      by tender joint count, swollen joint count, patient's global assessment of 
      disease activity, erythrocyte sedimentation rate (ESR), and levels of C-reactive 
      protein (CRP), matrix metalloproteinase-3 (MMP-3), and rheumatoid factor (RF), as 
      well as 28-joint count Disease Activity Score (DAS28). OPN levels in plasma and 
      urine were measured by ELISA. RESULTS: Median levels of thrombin-cleaved OPN in 
      urine (U-half) were significantly higher in RA patients (143.5 pmol/mmol Cr) than 
      in healthy controls (67.9 pmol/mmol Cr) or OA patients (69.8 pmol/mmol Cr). 
      Thrombin-cleaved OPN was not detected in plasma. U-half levels correlated 
      significantly with levels of CRP (r = 0.26, p = 0.03), ESR (r = 0.26, p = 0.03), 
      and RF (r = 0.28, p = 0.03). Median U-half levels were significantly higher in 
      patients with stage III (249.9 pmol/mmol Cr) and IV (251.6 pmol/mmol Cr) disease 
      than in patients with stage I (98.6 pmol/mmol Cr) disease. CONCLUSION: Our 
      results suggest that urine levels of the thrombin-cleaved isoform of OPN may 
      reflect the severity of active inflammatory arthritis in patients with RA.
FAU - Shio, Kiori
AU  - Shio K
AD  - Department of Internal Medicine II, Fukushima Medical University School of 
      Medicine, Hikarigaoka, Fukushima, Japan.
FAU - Kobayashi, Hiroko
AU  - Kobayashi H
FAU - Asano, Tomoyuki
AU  - Asano T
FAU - Saito, Rie
AU  - Saito R
FAU - Iwadate, Haruyo
AU  - Iwadate H
FAU - Watanabe, Hiroshi
AU  - Watanabe H
FAU - Sakuma, Hirofumi
AU  - Sakuma H
FAU - Segawa, Tetsuya
AU  - Segawa T
FAU - Maeda, Masahiro
AU  - Maeda M
FAU - Ohira, Hiromasa
AU  - Ohira H
LA  - eng
PT  - Journal Article
DEP - 20100215
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Protein Isoforms)
RN  - 106441-73-0 (Osteopontin)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 9009-79-4 (Rheumatoid Factor)
RN  - EC 3.4.21.5 (Thrombin)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Arthritis, Rheumatoid/blood/*urine
MH  - Blood Sedimentation
MH  - C-Reactive Protein/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Inflammation/blood/*urine
MH  - Male
MH  - Matrix Metalloproteinase 3/blood
MH  - Middle Aged
MH  - Osteoarthritis/blood/urine
MH  - Osteopontin/blood/*urine
MH  - Pain Measurement
MH  - Protein Isoforms/blood/urine
MH  - Rheumatoid Factor/blood
MH  - Severity of Illness Index
MH  - Thrombin/*metabolism
EDAT- 2010/02/17 06:00
MHDA- 2010/06/23 06:00
CRDT- 2010/02/17 06:00
PHST- 2010/02/17 06:00 [entrez]
PHST- 2010/02/17 06:00 [pubmed]
PHST- 2010/06/23 06:00 [medline]
AID - jrheum.090582 [pii]
AID - 10.3899/jrheum.090582 [doi]
PST - ppublish
SO  - J Rheumatol. 2010 Apr;37(4):704-10. doi: 10.3899/jrheum.090582. Epub 2010 Feb 15.