PMID- 20158696
OWN - NLM
STAT- MEDLINE
DCOM- 20101013
LR  - 20100610
IS  - 1423-0410 (Electronic)
IS  - 0042-9007 (Linking)
VI  - 98
IP  - 4
DP  - 2010 May
TI  - Stimulation of human neutrophils with sera containing HLA Class I alloantibody 
      causes preferential degranulation of azurophilic granules and secretory vesicles.
PG  - 560-6
LID - 10.1111/j.1423-0410.2010.01311.x [doi]
AB  - BACKGROUND AND OBJECTIVES: The activation of neutrophils by human leukocyte 
      antigen (HLA) Class I alloantibody is thought to be involved in 
      transfusion-related acute lung injury. Neutrophils contain various biological 
      substances in four groups of granules, including secretory vesicles, azurophilic 
      granules, specific granules and gelatinase granules. To characterize the 
      activation of neutrophils by HLA Class I alloantibody, we investigated whether 
      HLA Class I alloantibody could cause the degranulation of these groups of 
      granules either coordinately or selectively. MATERIALS AND METHODS: Sera 
      containing HLA-A24 alloantibody were incubated with neutrophils in a washed whole 
      blood system. CD11b expression (secretory vesicles) on neutrophils was analysed 
      by flow cytometry, and the secretion of markers of each granule was determined by 
      ELISA. RESULTS: The treatment of cross-matching-positive neutrophils with sera 
      containing HLA-A24 alloantibody caused the significant expression of CD11b, and 
      the significant secretion of neutrophil elastase and myeloperoxidase, azurophilic 
      granule markers and heparin-binding protein (HBP), which is localized in 
      secretory vesicles and azurophilic granules when compared with 
      cross-matching-negative neutrophils. In contrast, no significant differences were 
      observed in the secretion of lactoferrin, a marker of specific granules, and 
      matrix methalloproteinase-9, a marker of gelatinase granules between 
      cross-matching-positive and cross-matching-negative cells upon stimulation with 
      sera. CD11b expression and secretion of HBP by serum was partially inhibited by 
      p38 mitogen-activated protein (MAP)-kinase inhibitors. CONCLUSION: Neutrophils 
      activated with sera containing HLA Class I alloantibody caused the preferential 
      degranulation of azurophilic granules and secretory vesicles. This process was at 
      least in part mediated by p38 MAP kinase-involved signal transduction.
FAU - Takahashi, D
AU  - Takahashi D
AD  - Japanese Red Cross, Hokkaido Red Cross Blood Center, Sapporo, Japan.
FAU - Fujihara, M
AU  - Fujihara M
FAU - Azuma, H
AU  - Azuma H
FAU - Miyazaki, T
AU  - Miyazaki T
FAU - Uchimura, D
AU  - Uchimura D
FAU - Wakamoto, S
AU  - Wakamoto S
FAU - Sato, S
AU  - Sato S
FAU - Kato, T
AU  - Kato T
FAU - Ikeda, H
AU  - Ikeda H
LA  - eng
PT  - Journal Article
DEP - 20100211
PL  - England
TA  - Vox Sang
JT  - Vox sanguinis
JID - 0413606
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Cell Degranulation/immunology
MH  - Cytoplasmic Granules/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Histocompatibility Antigens Class I/blood/*immunology
MH  - Humans
MH  - Isoantibodies/*blood/immunology
MH  - Neutrophils/*immunology/metabolism
MH  - Secretory Vesicles/*immunology/metabolism
EDAT- 2010/02/18 06:00
MHDA- 2010/10/14 06:00
CRDT- 2010/02/18 06:00
PHST- 2010/02/18 06:00 [entrez]
PHST- 2010/02/18 06:00 [pubmed]
PHST- 2010/10/14 06:00 [medline]
AID - VOX1311 [pii]
AID - 10.1111/j.1423-0410.2010.01311.x [doi]
PST - ppublish
SO  - Vox Sang. 2010 May;98(4):560-6. doi: 10.1111/j.1423-0410.2010.01311.x. Epub 2010 
      Feb 11.