PMID- 20160469
OWN - NLM
STAT- MEDLINE
DCOM- 20110211
LR  - 20211020
IS  - 1720-8386 (Electronic)
IS  - 0391-4097 (Linking)
VI  - 33
IP  - 8
DP  - 2010 Sep
TI  - Comparison between immunoradiometric and fluorimetric brain natriuretic peptide 
      determination in patients with congestive heart failure.
PG  - 554-8
AB  - This study compared two different methods, namely the immunoradiometric (IRMA) 
      and fluorimetric (FIA), in order to determine plasma brain natriuretic peptide 
      (BNP) in congestive heart failure (CHF) patients. METHODS: CHF in-patients 
      underwent echocardiography and plasma BNP determination using both two methods. 
      The echocardiograms analysed left ventricular end-systolic (LVESV) and 
      end-diastolic (LVEDV) volumes and systolic dysfunction [left ventricular ejection 
      fraction (LVEF) <50%]. RESULTS: Seventy-three (71% males, age 67 +/- 9.6 yr) 
      patients were enrolled, 31.5% affected by valvular heart disease. The mean LVEF 
      was 39.8 +/- 14.1%; in 26 (35%) a hypertensive etiology emerged. The 
      immunoradiometric assay (IRMA) BNP was found to be significantly lower than the 
      FIA determination 116.5 +/- 149 pg/ml vs 267.3 +/- 285.6 pg/ml; p=0.0001) and the two 
      methods were closely correlated (r=0.89; p=0.00001). Logistic regression 
      demonstrated a significant correlation between BNP, LVEF, and LVESV/LVEDV 
      (r=-0.45, p=0.0003; r=-0.48, p=0.00001; r=0.22 p=0.003; r=0.34 p=0.0001; r=0.13 
      p=0.02; r=0.28 p=0.001 IRMA and FIA, respectively). IRMA BNP and FIA BNP 
      significantly increased according to the worsening functional class [from 34.3 +/- 
      60.2 pg/ml in NYHA (New York Heart Association) I to 555.5 +/- 273.1 pg/ml in NYHA 
      IV; from 86.1 +/- 162.1 pg/ml in NYHA I to 1070 +/- 42.2 pg/ml in NYHA IV, 
      respectively]. In severe systolic dysfunction (LVEF<30%), receiver operating 
      characteristic analysis revealed a satisfactorily sensitivity and specificity 
      using a cut-off point of 50.6 pg/ml with IRMA and 243 pg/ml with FIA. In mild 
      systolic dysfunction (LVEF<50%), a good sensitivity and specificity using a 
      cut-off point of 42 pg/ml with IRMA and 182 pg/ml with FIA emerged. CONCLUSIONS: 
      In CHF patients both BNP methods correlated with NYHA class, LVEF, and 
      ventricular volumes.
FAU - Feola, M
AU  - Feola M
AD  - Cardiovascular Rehabilitation-Heart Failure Unit, SS. Trinita Hospital, Via 
      Ospedale 4, 12045 Fossano, Italy. m_feola@virgilio.it
FAU - Valeri, L
AU  - Valeri L
FAU - Menditto, E
AU  - Menditto E
FAU - Nervo, E
AU  - Nervo E
FAU - Bianco, F
AU  - Bianco F
FAU - Aspromonte, N
AU  - Aspromonte N
FAU - Valle, R
AU  - Valle R
FAU - Visconti, G
AU  - Visconti G
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20100215
PL  - Italy
TA  - J Endocrinol Invest
JT  - Journal of endocrinological investigation
JID - 7806594
RN  - 114471-18-0 (Natriuretic Peptide, Brain)
SB  - IM
MH  - Aged
MH  - Echocardiography
MH  - Female
MH  - *Fluoroimmunoassay
MH  - Heart Failure/*blood
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Natriuretic Peptide, Brain/*analysis
MH  - *Radioimmunoassay
MH  - Sensitivity and Specificity
MH  - Stroke Volume
MH  - Ventricular Dysfunction, Left/diagnostic imaging
EDAT- 2010/02/18 06:00
MHDA- 2011/02/12 06:00
CRDT- 2010/02/18 06:00
PHST- 2010/02/18 06:00 [entrez]
PHST- 2010/02/18 06:00 [pubmed]
PHST- 2011/02/12 06:00 [medline]
AID - 6865 [pii]
AID - 10.1007/BF03346647 [doi]
PST - ppublish
SO  - J Endocrinol Invest. 2010 Sep;33(8):554-8. doi: 10.1007/BF03346647. Epub 2010 Feb 
      15.