PMID- 2016124
OWN - NLM
STAT- MEDLINE
DCOM- 19910517
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 72
IP  - 2
DP  - 1991 Feb
TI  - Generation of a long-lived IgE response in high and low responder strains of rat 
      by co-administration of ricin and antigen.
PG  - 297-303
AB  - Certain strains of rats infested with the nematode parasite Nippostrongulus 
      brasiliensis mount vigorous, persistent immunoglobulin E (IgE) responses. In the 
      absence of parasites, adjuvants such as Bordatella pertussis or Al(OH)3 are 
      needed to produce IgE responses to soluble antigens. These are short-lived, even 
      in high IgE responder strains. In this study we have produced long-lived IgE 
      responses in both low (Wistar) and high (Brown Norway) IgE responder strains of 
      rats by repeated injections of ricin, a toxic lectin from castor beans, and 
      phospholipase A2 (PLA2), a bee venom protein. Total IgE levels rose from 30 +/- 
      20 ng/ml to 39,000 +/- 7500 ng/ml in the Wistar rats compared with an increase 
      from 120 +/- 100 ng/ml to 47,000 +/- 8000 ng/ml in the Brown Norway rats. An even 
      greater (10(4)-fold) increase was seen in PLA2-specific IgE antibody levels. 
      total and PLA2-specific IgE started to fall 6 weeks after treatment was stopped 
      in the Wistar and after 12 weeks in the Brown Norway rats. The duration of the 
      response was 204 and 248 days, respectively. The IgE-enhancing properties of 
      ricin were compared in low, mid (Hooded Lister) and high IgE responder rats. 
      Total IgE and PLA2-specific IgE but not IgG antibody (Ab) responses were enhanced 
      in all animals given ricin and PLA2 but not in animals given ricin or PLA2 alone. 
      The increase was greater in Wistar rats (48-fold) than in Brown Norway rats 
      (eightfold) and by Day 24 the levels of both total and PLA2-specific IgE in three 
      different strains were indistinguishable. PLA2-specific IgE antibody-secreting 
      cells were detected in the spleen at a frequency of 1:5000. These results show: 
      (i) that repeated immunization of rats with antigen and ricin produce a very 
      large IgE response which was long-lived; (ii) that this response was 
      indistinguishable in different IgE responder strains of rat; and (iii) that the 
      IgE response declines earlier in low IgE responder strains of rats.
FAU - Diaz-Sanchez, D
AU  - Diaz-Sanchez D
AD  - Department of Allergy and Allied Respiratory Disorders, United Medical School, 
      Guy's Hospital, London.
FAU - Kemeny, D M
AU  - Kemeny DM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antigens)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9009-86-3 (Ricin)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
SB  - IM
MH  - *Adjuvants, Immunologic
MH  - Animals
MH  - Antibody-Producing Cells/immunology
MH  - Antigens/*immunology
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Phospholipases A/immunology
MH  - Phospholipases A2
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Inbred Strains
MH  - Ricin/*immunology
MH  - Spleen/immunology
MH  - Time Factors
PMC - PMC1384500
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
PMCR- 1992/02/01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
PHST- 1992/02/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1991 Feb;72(2):297-303.