PMID- 20163519
OWN - NLM
STAT- MEDLINE
DCOM- 20101005
LR  - 20100611
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 22
IP  - 5
DP  - 2010 May
TI  - Electrophysiological identification of the functional presynaptic nerve terminals 
      on an isolated single vasopressin neurone of the rat supraoptic nucleus.
PG  - 413-9
LID - 10.1111/j.1365-2826.2010.01979.x [doi]
AB  - Release of arginine vasopressin (AVP) and oxytocin from magnocellular 
      neurosecretory cells (MNCs) of the supraoptic nucleus (SON) is under the control 
      of glutamate-dependent excitation and GABA-dependent inhibition. The possible 
      role of the synaptic terminals attached to SON neurones has been investigated 
      using whole-cell patch-clamp recording in in vitro rat brain slice preparations. 
      Recent evidence has provided new insights into the repercussions of glial 
      environment modifications on the physiology of MNCs at the synaptic level in the 
      SON. In the present study, excitatory glutamatergic and inhibitory GABAergic 
      synaptic inputs were recorded from an isolated single SON neurone cultured for 12 
      h, using the whole-cell patch clamp technique. Neurones expressed an AVP-enhanced 
      green fluorescent protein (eGFP) fusion gene in MNCs. In addition, native 
      synaptic terminals attached to a dissociated AVP-eGFP neurone were visualised 
      with synaptic vesicle markers. These results suggest that the function of 
      presynaptic nerve terminals may be evaluated directly in a single AVP-eGFP 
      neurone. These preparations would be helpful in future studies aiming to 
      electrophysiologically distinguish between the functions of synaptic terminals 
      and glial modifications in the SON neurones.
FAU - Ohbuchi, T
AU  - Ohbuchi T
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu 807-8555, Japan.
FAU - Yokoyama, T
AU  - Yokoyama T
FAU - Fujihara, H
AU  - Fujihara H
FAU - Suzuki, H
AU  - Suzuki H
FAU - Ueta, Y
AU  - Ueta Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100212
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Arginine Vasopressin/genetics/*physiology
MH  - Green Fluorescent Proteins/genetics
MH  - Male
MH  - Neurons/*physiology
MH  - Patch-Clamp Techniques
MH  - Presynaptic Terminals/*physiology
MH  - Rats
MH  - Rats, Transgenic
MH  - Supraoptic Nucleus/*physiology
EDAT- 2010/02/19 06:00
MHDA- 2010/10/06 06:00
CRDT- 2010/02/19 06:00
PHST- 2010/02/19 06:00 [entrez]
PHST- 2010/02/19 06:00 [pubmed]
PHST- 2010/10/06 06:00 [medline]
AID - JNE1979 [pii]
AID - 10.1111/j.1365-2826.2010.01979.x [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2010 May;22(5):413-9. doi: 10.1111/j.1365-2826.2010.01979.x. 
      Epub 2010 Feb 12.