PMID- 20164046
OWN - NLM
STAT- MEDLINE
DCOM- 20101103
LR  - 20151119
IS  - 1460-2385 (Electronic)
IS  - 0931-0509 (Linking)
VI  - 25
IP  - 7
DP  - 2010 Jul
TI  - Plasma and urine leukocyte elastase-alpha1protease inhibitor complex as a marker 
      of early and long-term kidney graft function.
PG  - 2346-51
LID - 10.1093/ndt/gfq041 [doi]
AB  - BACKGROUND: Neutrophils are mediators of ischaemia/reperfusion (I/R) injury 
      following kidney transplantation (kTx). Leukocyte elastase (LE) complex with 
      alpha(1)protease inhibitor (LE-alpha(1)PI) is a marker of neutrophil 
      degranulation. The aim of this study was to evaluate LE-alpha(1)PI as a marker of 
      I/R kidney damage and to search for correlations between leukocyte activation and 
      post-transplant complications. METHODS: Plasma and urine LE-alpha(1)PI were 
      estimated in 55 deceased-donor kidney graft recipients on postoperative days 
      (POD) 1, 3 and 7, as well as in the late post-transplant period. RESULTS: The 
      plasma LE-alpha(1)PI level peaked on POD 1 after kTx, and the urine LE-alpha(1)PI 
      peaked on POD 3. On POD 1 and POD 3, the urine LE-alpha(1)PI levels were higher 
      in delayed graft function (DGF) patients than in patients with immediate graft 
      function (IGF: P < 0.001 and P < 0.003, respectively). Urine LE-alpha(1)PI 
      excretion on POD 1 was significantly higher in patients with longer cold 
      ischaemia time (CIT) than in patients with shorter CIT, P < 0.002. Multivariate 
      regression model revealed two factors influencing the occurrence of early acute 
      rejection-urine LE-alpha(1)PI complex on POD 3 and human leukocyte antigen (HLA) 
      mismatches. There was a significant association between the plasma LE-alpha(1)PI 
      on POD 3 and serum creatinine level 6 and 12 months after kTx (r(2) 0.24; P < 
      0.005 and 0.19; P < 0.005, respectively). CONCLUSIONS: This study is the first 
      presentation of a simple, non-invasive measurement of neutrophil activation after 
      kTx. It also demonstrates a strong correlation between the early post-transplant 
      LE-alpha(1)PI complex level and kidney graft function.
FAU - Zynek-Litwin, Malgorzata
AU  - Zynek-Litwin M
AD  - Department of Nephrology and Transplantation Medicine, Wroclaw Medical 
      University, Wroclaw, Poland. malgorzata.litwin@gmail.com
FAU - Kuzniar, Jakub
AU  - Kuzniar J
FAU - Marchewka, Zofia
AU  - Marchewka Z
FAU - Kopec, Waclaw
AU  - Kopec W
FAU - Kusztal, Mariusz
AU  - Kusztal M
FAU - Patrzalek, Dariusz
AU  - Patrzalek D
FAU - Biecek, Przemyslaw
AU  - Biecek P
FAU - Klinger, Marian
AU  - Klinger M
LA  - eng
PT  - Journal Article
DEP - 20100216
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Biomarkers)
RN  - 0 (Protease Inhibitors)
RN  - 0 (alpha 1-Antitrypsin)
RN  - EC 3.4.21.37 (Leukocyte Elastase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers/blood/urine
MH  - Female
MH  - Graft Survival/*physiology
MH  - Humans
MH  - Kidney Transplantation/*physiology
MH  - Leukocyte Elastase/*blood/*urine
MH  - Male
MH  - Middle Aged
MH  - Multivariate Analysis
MH  - Neutrophil Activation
MH  - Predictive Value of Tests
MH  - Protease Inhibitors/*blood/*urine
MH  - Retrospective Studies
MH  - alpha 1-Antitrypsin/blood/urine
EDAT- 2010/02/19 06:00
MHDA- 2010/11/04 06:00
CRDT- 2010/02/19 06:00
PHST- 2010/02/19 06:00 [entrez]
PHST- 2010/02/19 06:00 [pubmed]
PHST- 2010/11/04 06:00 [medline]
AID - gfq041 [pii]
AID - 10.1093/ndt/gfq041 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2010 Jul;25(7):2346-51. doi: 10.1093/ndt/gfq041. Epub 
      2010 Feb 16.