PMID- 20167546
OWN - NLM
STAT- MEDLINE
DCOM- 20100514
LR  - 20191210
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 878
IP  - 9-10
DP  - 2010 Mar 15
TI  - Gas chromatography-ion trap mass spectrometry method for the simultaneous 
      measurement of MDMA (ecstasy) and its metabolites, MDA, HMA, and HMMA in plasma 
      and urine.
PG  - 815-22
LID - 10.1016/j.jchromb.2010.01.042 [doi]
AB  - The investigation of 3,4-methylenedioxymethamphetamine (MDMA; ecstasy) abuse 
      requires very robust methods with high sensitivity and wide linearity ranges for 
      the quantification of this drug of abuse and its main metabolites in body fluids. 
      An optimized gas chromatography-ion trap mass spectrometry (GC-IT/MS) methodology 
      with electron impact ionization addressing these issues is presented. The sample 
      preparation involves an enzymatic hydrolysis of urine and plasma for conjugate 
      cleavage, a SPE extraction, and a derivatization process. The method was fully 
      validated in rat plasma and urine. Linearity for a wide concentration range was 
      achieved for MDMA, and the metabolites 3,4-methylenedioxyamphetamine (MDA), 
      4-hydroxy-3-methoxyamphetamine (HMA) and 4-hydroxy-3-methoxymethamphetamine 
      (HMMA). Limits of quantification were 2 ng/mL in plasma and 3.5 ng/mL in urine 
      using a Selected Ion Monitoring detection mode. Selectivity, accuracy, precision, 
      and recovery met the required criteria for the method validation. This GC-IT/MS 
      method provides high sensitivity and adequate performance characteristics for the 
      simultaneous quantification of MDMA, MDA, HMA and HMMA in the studied matrices.
CI  - 2010 Elsevier B.V. All rights reserved.
FAU - da Silva, Daniel Gomes
AU  - da Silva DG
AD  - REQUIMTE, Toxicology Department, Faculty of Pharmacy, University of Porto, Rua 
      Anibal Cunha 164, 4099-030 Porto, Portugal.
FAU - de Pinho, Paula Guedes
AU  - de Pinho PG
FAU - Pontes, Helena
AU  - Pontes H
FAU - Ferreira, Luisa
AU  - Ferreira L
FAU - Branco, Paula
AU  - Branco P
FAU - Remiao, Fernando
AU  - Remiao F
FAU - Carvalho, Felix
AU  - Carvalho F
FAU - Bastos, M Lurdes
AU  - Bastos ML
FAU - Carmo, Helena
AU  - Carmo H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100204
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 117652-28-5 (4-hydroxy-3-methoxymethamphetamine)
RN  - 13026-44-3 (3-O-methyl-alpha-methyldopamine)
RN  - 44RAL3456C (Methamphetamine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*analysis/blood/urine
MH  - Animals
MH  - Dopamine/*analogs & derivatives/analysis/blood/urine
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Male
MH  - Methamphetamine/*analogs & derivatives/analysis/blood/urine
MH  - N-Methyl-3,4-methylenedioxyamphetamine/blood/*metabolism/urine
MH  - Rats
MH  - Rats, Wistar
MH  - Sensitivity and Specificity
EDAT- 2010/02/20 06:00
MHDA- 2010/05/15 06:00
CRDT- 2010/02/20 06:00
PHST- 2009/12/10 00:00 [received]
PHST- 2010/01/25 00:00 [revised]
PHST- 2010/01/27 00:00 [accepted]
PHST- 2010/02/20 06:00 [entrez]
PHST- 2010/02/20 06:00 [pubmed]
PHST- 2010/05/15 06:00 [medline]
AID - S1570-0232(10)00064-4 [pii]
AID - 10.1016/j.jchromb.2010.01.042 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Mar 15;878(9-10):815-22. doi: 
      10.1016/j.jchromb.2010.01.042. Epub 2010 Feb 4.