PMID- 20169940
OWN - NLM
STAT- MEDLINE
DCOM- 20100420
LR  - 20151119
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 58
IP  - 1
DP  - 2010 Jan
TI  - [Comparison of two HER-2 FISH kits on formalin-fixed paraffin-embedded tissues: 
      signal detection and simple procedure].
PG  - 25-9
AB  - The gene amplification of human epidermal growth factor receptor (HER-2) detected 
      by fluorescence in situ hybridization (FISH) help to select breast cancer 
      patients who could benefit from therapeutic strategies targeting HER-2, 
      trastuzumab. For formalin-fixed paraffin-embedded tissue specimens, the 
      preprocessing procedure, which is consisted of heat-treatment and protease 
      digestion, is necessary to detect probe specific signal by FISH. We compared the 
      findings of two commercial kits for HER-2 (Histra HER2 FISH Kit [JOKOH CO.LTD] 
      and Pathvysion HER-2 DNA Probe Kit [Abbot Molecular CO. LTD]) using 9 breast 
      cancer specimens, which were fixed in formalin for various durations after the 
      surgical extirpation or biopsy. The two kits showed the same results that 
      HER-2/17 centromere (17cen) signal ratio was within normal rage (< 2.0) in 7 
      cases among 9 and was over 2.0 in remaining 2. Histra could obtain sufficient 
      signals on both surgical and needle biopsy speciments after the same 
      pretreatments. PathVysion, on the other hand, require a different protease or 
      modification of protease digestion time. Furthermore, the specific signals for 
      HER-2 and 17cen could be observed more clearly by Histra than PathVysion under 
      fluorescensce microscope. Histra is more suitable for routine examination because 
      of its simple and constant-processing procedure.
FAU - Uhara, Miho
AU  - Uhara M
AD  - Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto 
      390-8621, Japan.
FAU - Matsuda, Kazuyuki
AU  - Matsuda K
FAU - Shimojyo, Yasuyo
AU  - Shimojyo Y
FAU - Hidaka, Eiko
AU  - Hidaka E
FAU - Kumagai, Toshiko
AU  - Kumagai T
FAU - Uehara, Takeshi
AU  - Uehara T
FAU - Sano, Kenji
AU  - Sano K
FAU - Honda, Takayuki
AU  - Honda T
LA  - jpn
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Biomarkers, Tumor/*analysis
MH  - Breast Neoplasms/diagnosis
MH  - Female
MH  - *Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Paraffin Embedding
MH  - *Reagent Kits, Diagnostic
MH  - Receptor, ErbB-2/*analysis
EDAT- 2010/02/23 06:00
MHDA- 2010/04/21 06:00
CRDT- 2010/02/23 06:00
PHST- 2010/02/23 06:00 [entrez]
PHST- 2010/02/23 06:00 [pubmed]
PHST- 2010/04/21 06:00 [medline]
PST - ppublish
SO  - Rinsho Byori. 2010 Jan;58(1):25-9.