PMID- 20170082
OWN - NLM
STAT- MEDLINE
DCOM- 20100608
LR  - 20191210
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 82
IP  - 6
DP  - 2010 Mar 15
TI  - Automated needle trap heart-cut GC/MS and needle trap comprehensive 
      two-dimensional GC/TOF-MS for breath gas analysis in the clinical environment.
PG  - 2541-51
LID - 10.1021/ac100061k [doi]
AB  - This study was intended to evaluate low-volume (20 mL) multibed needle trap (NTD) 
      sampling combined with heart-cut gas chromatography/mass spectrometry (GC/MS) and 
      comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry 
      (GC x GC/TOF-MS) for trace gas analysis under clinical conditions. NTDs, 
      high-throughput automatic desorption and separation systems, were tested in vitro 
      and within a study in 11 patients undergoing cardiac surgery with respect to 
      reproducibility, reliability, and clinical applicability. NTD-heart-cut GC/MS 
      analysis of standard mixtures containing different volatile organic compounds 
      (VOCs) yielded relative standard deviations (RSDs) from 4.0% to 18.5%. Substance 
      adsorption was stable for 1 day if NTDs were closed on both ends and was stable 
      for approximately 7.8 h when NTD tip ends had to be left open during autosampler 
      storage. Even in the presence of high concentrations of contaminants linearity of 
      heart-cut GC/MS was conserved. In patients' breath potential biomarkers could be 
      determined even in the presence of very high concentrations of sevoflurane. 
      Profiles of blood-borne biomarkers, intravenous drugs, and clinical contaminants 
      were characterized. Comprehensive GC x GC/TOF-MS may be used as a screening tool 
      for new biomarkers, if patterns are generated from deconvoluted normalized areas. 
      Needle trap sampling in combination with hyphenated chromatographic techniques 
      can thus be used to provide well-tailored solutions for complex problems 
      occurring in clinical breath analysis.
FAU - Mieth, Maren
AU  - Mieth M
AD  - Department of Anesthesiology and Intensive Care Medicine, University Rostock, 
      Schillingallee 35, 18057 Rostock, Germany.
FAU - Schubert, Jochen K
AU  - Schubert JK
FAU - Groger, Thomas
AU  - Groger T
FAU - Sabel, Bastian
AU  - Sabel B
FAU - Kischkel, Sabine
AU  - Kischkel S
FAU - Fuchs, Patricia
AU  - Fuchs P
FAU - Hein, Dietmar
AU  - Hein D
FAU - Zimmermann, Ralf
AU  - Zimmermann R
FAU - Miekisch, Wolfram
AU  - Miekisch W
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Gases)
SB  - IM
MH  - Breath Tests/*instrumentation/methods
MH  - Equipment Design
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Gases/*analysis
MH  - Humans
MH  - Linear Models
MH  - Pulmonary Alveoli/*chemistry
MH  - Reproducibility of Results
EDAT- 2010/02/23 06:00
MHDA- 2010/06/09 06:00
CRDT- 2010/02/23 06:00
PHST- 2010/02/23 06:00 [entrez]
PHST- 2010/02/23 06:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
AID - 10.1021/ac100061k [doi]
PST - ppublish
SO  - Anal Chem. 2010 Mar 15;82(6):2541-51. doi: 10.1021/ac100061k.