PMID- 20170319
OWN - NLM
STAT- MEDLINE
DCOM- 20100405
LR  - 20151119
IS  - 1532-4303 (Electronic)
IS  - 0277-0903 (Linking)
VI  - 47
IP  - 2
DP  - 2010 Mar
TI  - Nitric oxide in both bronchoalveolar lavage fluid and serum is associated with 
      pathogenesis and severity of antigen-induced pulmonary inflammation in rats.
PG  - 135-44
LID - 10.3109/02770900903483808 [doi]
AB  - BACKGROUND: Nitric oxide (NO) is considered as a hallmark for allergic airway 
      inflammation in asthmatics and animal models. But the correlation between NO and 
      antigen-induced pulmonary inflammation (AIPI), a rat model for asthma, in varying 
      genetic background population has not been completely understood. OBJECTIVE: The 
      objective in this study is to observe the different responsiveness to AIPI in two 
      commonly used inbred rat strains and verify the correlation between NO from 
      different sources and pathological parameters of AIPI by using Dark Agouti (DA), 
      E3, F1 (E3 x DA), and F2 rat populations. METHODS: AIPI was induced by 
      systemically immunizing and intranasally challenging E3, DA, F1 (DA x E3), and F2 
      rats with ovalbumin (OVA). Pathological changes and mucus secretion in lungs were 
      observed after hematoxylin and eosin (HE) and periodic acid Schiff (PAS) 
      staining, whereas eosinophils in bronchoalveolar lavage fluid (BALF) were counted 
      after Giemsa staining. Delayed-type hyperresponsiveness was determined by 
      subcutaneous injection of OVA in ear. Total immunoglobulin E (IgE) and 
      OVA-specific IgG1 were detected with enzyme-linked immunosorbent assay (ELISA). 
      NO concentration was measured by the Griess method. RESULTS: DA rats were 
      unresponsive to OVA treatment, whereas E3 rats were susceptible to AIPI. F1 rats 
      manifested the same responsiveness to OVA treatment as DA rats, and individual F2 
      rats showed the variable severity of AIPI. NO concentration in BALF and serum was 
      significantly elevated in E3 rats but not in DA and F1 rats after OVA treatment. 
      In F2 rats, NO concentration in serum was positively correlated with eosinophils 
      in BALF, total IgE, and pathological scores, whereas NO concentration in BALF 
      correlated only with eosinophils in BALF and total IgE. CONCLUSION: DA and F1 
      rats are resistant, whereas E3 rats are sensitive, to AIPI. NO in serum can 
      represent the severity of allergic inflammation and pathological changes in lungs 
      in F2 population.
FAU - Yang, Xudong
AU  - Yang X
AD  - Department of Genetics and Molecular Biology, Xi'an Jiaotong University School of 
      Medicine, Xir'an, Shaanxi 710061, PR China.
FAU - Sun, Qingzhu
AU  - Sun Q
FAU - Asim, M B Raza
AU  - Asim MB
FAU - Jiang, Xiaogang
AU  - Jiang X
FAU - Zhong, Bo
AU  - Zhong B
FAU - Shahzad, Muhammad
AU  - Shahzad M
FAU - Zhang, Fujun
AU  - Zhang F
FAU - Han, Yan
AU  - Han Y
FAU - Lu, Shemin
AU  - Lu S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Asthma
JT  - The Journal of asthma : official journal of the Association for the Care of 
      Asthma
JID - 8106454
RN  - 0 (Antigens)
RN  - 0 (Biomarkers)
RN  - 0 (Immunoglobulin G)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antigens/*immunology
MH  - Asthma/genetics/immunology/*metabolism/pathology
MH  - Biomarkers/analysis/blood/metabolism
MH  - Bronchoalveolar Lavage Fluid/*chemistry
MH  - Disease Models, Animal
MH  - Eosinophilia/pathology
MH  - Female
MH  - Hybridization, Genetic
MH  - Hypersensitivity, Delayed/immunology
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood/immunology
MH  - Inflammation/genetics/immunology/metabolism/pathology
MH  - Lung/metabolism/pathology
MH  - Male
MH  - Nitric Oxide/analysis/*blood/*metabolism
MH  - Ovalbumin/immunology
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sex Characteristics
MH  - Vaccination
EDAT- 2010/02/23 06:00
MHDA- 2010/04/07 06:00
CRDT- 2010/02/23 06:00
PHST- 2010/02/23 06:00 [entrez]
PHST- 2010/02/23 06:00 [pubmed]
PHST- 2010/04/07 06:00 [medline]
AID - 10.3109/02770900903483808 [doi]
PST - ppublish
SO  - J Asthma. 2010 Mar;47(2):135-44. doi: 10.3109/02770900903483808.