PMID- 20171071
OWN - NLM
STAT- MEDLINE
DCOM- 20101207
LR  - 20161125
IS  - 1618-095X (Electronic)
IS  - 0944-7113 (Linking)
VI  - 17
IP  - 10
DP  - 2010 Aug
TI  - Protective effects of Asiaticoside on acute liver injury induced by 
      lipopolysaccharide/D-galactosamine in mice.
PG  - 811-9
LID - 10.1016/j.phymed.2010.01.008 [doi]
AB  - Asiaticoside (AS), a triterpenoid product isolated from Centella asiatica, has 
      been described to exhibit anti-in fl ammatory activities in several inflammatory 
      models. However, the effects of AS on liver injury are poorly understood. The 
      present study was undertaken to investigate whether AS is efficacious against 
      Lipopolysaccharide (LPS) /D-galactosamine (D-GalN)-induced acute liver injury in 
      mice and its potential mechanisms. AS (5, 10 and 20 mg/kg/d) was pretreated 
      orally once daily for 3 days before LPS/D-GalN injected in mice. The mortality, 
      hepatic tissue histology, plasma levels of Tumor necrosis factor-alpha 
      (TNF-alpha) and alanine aminotransferase (ALT) and aspartate aminotransferase 
      (AST), hepatic tissue TNF-alpha and caspase-3 activity were measured. Besides, 
      western blotting analysis of phospho-p38 mitogen-activated protein kinase 
      (phospho-p38 MAPK), phospho-c-jun N-terminal kinase (phospho-JNK) and 
      phospho-extracellular signal regulated kinase (phospho-ERK) were determined. As a 
      result, AS showed significant protection as evidenced by the decrease of elevated 
      aminotransferases, hepatocytes apoptosis and caspase-3, alleviation of mortality 
      and improvement of liver pathological injury in a dose-dependent manner. Further, 
      we found that AS dose-dependently reduced the elevation of phospho-p38 MAPK, 
      phospho-JNK, phospho-ERK protein and TNF-alpha mRNA expression in liver tissues 
      and plasma TNF-alpha. These results suggest that AS has remarkable 
      hepatoprotective effects on LPS/D-GalN-induced liver injury and the possible 
      mechanism is related to inhibition of TNF-alpha and MAPKs.
CI  - 2010 Elsevier GmbH. All rights reserved.
FAU - Zhang, Li
AU  - Zhang L
AD  - Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing 
      Medical University, Chongqing 400016, People's Republic of China.
FAU - Li, Hong-Zhong
AU  - Li HZ
FAU - Gong, Xia
AU  - Gong X
FAU - Luo, Fu-Ling
AU  - Luo FL
FAU - Wang, Bin
AU  - Wang B
FAU - Hu, Ning
AU  - Hu N
FAU - Wang, Chang-Dong
AU  - Wang CD
FAU - Zhang, Zhuo
AU  - Zhang Z
FAU - Wan, Jing-Yuan
AU  - Wan JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100218
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (DNA Primers)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Triterpenes)
RN  - 7535-00-4 (Galactosamine)
RN  - PKO39VY215 (asiaticoside)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - Chemical and Drug Induced Liver Injury/*prevention & control
MH  - DNA Primers
MH  - Galactosamine/*toxicity
MH  - Lipopolysaccharides/*toxicity
MH  - MAP Kinase Signaling System
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Polymerase Chain Reaction
MH  - Triterpenes/*pharmacology
EDAT- 2010/02/23 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/02/23 06:00
PHST- 2009/09/18 00:00 [received]
PHST- 2009/11/05 00:00 [revised]
PHST- 2010/01/19 00:00 [accepted]
PHST- 2010/02/23 06:00 [entrez]
PHST- 2010/02/23 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S0944-7113(10)00024-3 [pii]
AID - 10.1016/j.phymed.2010.01.008 [doi]
PST - ppublish
SO  - Phytomedicine. 2010 Aug;17(10):811-9. doi: 10.1016/j.phymed.2010.01.008. Epub 
      2010 Feb 18.