PMID- 20171308
OWN - NLM
STAT- MEDLINE
DCOM- 20100903
LR  - 20220321
IS  - 1522-9653 (Electronic)
IS  - 1063-4584 (Print)
IS  - 1063-4584 (Linking)
VI  - 18
IP  - 5
DP  - 2010 May
TI  - Donor sex and age influence the chondrogenic potential of human femoral bone 
      marrow stem cells.
PG  - 705-13
LID - 10.1016/j.joca.2010.01.011 [doi]
AB  - OBJECTIVE: Damaged articular cartilage does not heal well and can progress to 
      osteoarthritis (OA). Human bone marrow stem cells (BMC) are promising cells for 
      articular cartilage repair, yet age- and sex-related differences in their 
      chondrogenesis have not been clearly identified. The purpose of this study is to 
      test whether the chondrogenic potential of human femoral BMC varies based on the 
      sex and/or age of the donor. DESIGN: BMC were isolated from 21 males (16-82 years 
      old (y.o.)) and 20 females (20-77 y.o.) during orthopaedic procedures. Cumulative 
      population doubling (CPD) was measured and chondrogenesis was evaluated by 
      standard pellet culture assay in the presence or absence of transforming growth 
      factor beta 1 (TGFbeta1). Pellet area was measured, and chondrogenic 
      differentiation was determined by Toluidine blue and Safranin O-Fast green 
      histological grading using the Bern score and by glycosaminoglycan (GAG) content. 
      RESULTS: No difference in CPD was observed due to donor sex or age. The increase 
      in pellet area with addition of TGFbeta1 and the Bern score significantly 
      decreased with increasing donor age in male BMC, but not in female BMC. A 
      significant reduction in GAG content per pellet was also observed with increasing 
      donor age in male BMC. This was not observed in female BMC. CONCLUSIONS: This 
      study showed an age-related decline in chondroid differentiation with TGFbeta1 
      stimulation in male BMC, but not in female BMC. Understanding the mechanisms for 
      these differences will contribute to improved clinical use of autologous BMC for 
      articular cartilage repair, and may lead to the development of customized age- or 
      sex-based treatments to delay or prevent the onset of OA.
CI  - Copyright 2010 Osteoarthritis Research Society International. Published by 
      Elsevier Ltd. All rights reserved.
FAU - Payne, K A
AU  - Payne KA
AD  - Cartilage Restoration Center, Department of Orthopaedic Surgery, University of 
      Pittsburgh, Pittsburgh, PA 15213, USA.
FAU - Didiano, D M
AU  - Didiano DM
FAU - Chu, C R
AU  - Chu CR
LA  - eng
GR  - R01 AR051963/AR/NIAMS NIH HHS/United States
GR  - R01 AR051963-01A2/AR/NIAMS NIH HHS/United States
GR  - R01 AR051963-03/AR/NIAMS NIH HHS/United States
GR  - 1 RO1 AR051963/AR/NIAMS NIH HHS/United States
GR  - R01 AR051963-02/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100206
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (Glycosaminoglycans)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow Cells/chemistry/cytology/*drug effects
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Chondrogenesis/*drug effects/physiology
MH  - Female
MH  - Femur/cytology
MH  - Glycosaminoglycans/analysis
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Sex Factors
MH  - Young Adult
PMC - PMC2862807
MID - NIHMS177864
COIS- Conflict of interest statement: The authors have no conflict of interest.
EDAT- 2010/02/23 06:00
MHDA- 2010/09/04 06:00
PMCR- 2011/05/01
CRDT- 2010/02/23 06:00
PHST- 2009/07/24 00:00 [received]
PHST- 2009/11/12 00:00 [revised]
PHST- 2010/01/04 00:00 [accepted]
PHST- 2010/02/23 06:00 [entrez]
PHST- 2010/02/23 06:00 [pubmed]
PHST- 2010/09/04 06:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - S1063-4584(10)00041-5 [pii]
AID - 10.1016/j.joca.2010.01.011 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2010 May;18(5):705-13. doi: 10.1016/j.joca.2010.01.011. 
      Epub 2010 Feb 6.