PMID- 20172426
OWN - NLM
STAT- MEDLINE
DCOM- 20100810
LR  - 20100222
IS  - 1578-1852 (Electronic)
IS  - 0213-005X (Linking)
VI  - 28 Suppl 1
DP  - 2010 Jan
TI  - [A review on new commercial methods for HIV-1 and HCV viral load determinations].
PG  - 62-7
LID - 10.1016/S0213-005X(10)70011-2 [doi]
AB  - Viral load techniques are more and more demanded in Clinical Microbiology, 
      regarding with transplanted patients or long time follow-up of chronic diseases 
      as those caused by human inmunodeficiency (HIV) and hepatitis C (HCV) viruses. In 
      the last 2 years, pharmaceutical companies, interested to develop more efficient 
      and accurate methods for the diagnosis and correct viral quantification of HIV 
      and HCV, have converged in the real time-polymerase chain reaction (PCR) 
      technique. This process is due to the increased sensitiviy, accuracy, linearity 
      and correct detection of genomic viral variants of real time PCR techniques, in 
      comparison with classical molecular methods applied since the nineties of the 
      past century. In spite real time PCR appears as the best tool for the immediate 
      future, new questions regarding the high variability of these viruses should be 
      considered. This could affect the correctness of viral quantifications, while 
      being difficult to detect it because of the methodological uniformity in the 
      clinical laboratories.
CI  - 2010 Elsevier Espana S.L. All rights reserved.
FAU - Aguilera, Antonio
AU  - Aguilera A
AD  - Complejo Hospitalario Universitario de Santiago, Santiago de Compostela, Espana. 
      antonio.aguilera.guirao@sergas.es <antonio.aguilera.guirao@sergas.es>
FAU - Alba, Jose Maria Gonzalez
AU  - Alba JM
FAU - Lamas, Lucia Martinez
AU  - Lamas LM
FAU - Suarez, Maria Luz Moldes
AU  - Suarez ML
FAU - Galan, Juan Carlos
AU  - Galan JC
LA  - spa
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Review
TT  - Evaluacion critica de los nuevos metodos comerciales para la determinacion de la 
      carga viral del VIH-1 y del VHC.
PL  - Spain
TA  - Enferm Infecc Microbiol Clin
JT  - Enfermedades infecciosas y microbiologia clinica
JID - 9104081
RN  - 0 (RNA, Viral)
SB  - IM
MH  - *Computer Systems
MH  - HIV Infections/blood/*virology
MH  - HIV-1/genetics/*isolation & purification
MH  - Hepacivirus/genetics/*isolation & purification
MH  - Hepatitis C/blood/*virology
MH  - Humans
MH  - Infectious Disease Medicine/organization & administration
MH  - Microbiology/organization & administration
MH  - Polymerase Chain Reaction/*methods
MH  - Quality Control
MH  - RNA, Viral/blood
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Societies, Medical/standards
MH  - Spain
MH  - *Viral Load
MH  - Viremia/*virology
MH  - Virology/*methods
RF  - 55
EDAT- 2010/02/23 06:00
MHDA- 2010/08/11 06:00
CRDT- 2010/02/23 06:00
PHST- 2010/02/23 06:00 [entrez]
PHST- 2010/02/23 06:00 [pubmed]
PHST- 2010/08/11 06:00 [medline]
AID - S0213-005X(10)70011-2 [pii]
AID - 10.1016/S0213-005X(10)70011-2 [doi]
PST - ppublish
SO  - Enferm Infecc Microbiol Clin. 2010 Jan;28 Suppl 1:62-7. doi: 
      10.1016/S0213-005X(10)70011-2.