PMID- 20173065
OWN - NLM
STAT- MEDLINE
DCOM- 20100615
LR  - 20211020
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 76
IP  - 8
DP  - 2010 Apr
TI  - Rickettsia symbionts cause parthenogenetic reproduction in the parasitoid wasp 
      Pnigalio soemius (Hymenoptera: Eulophidae).
PG  - 2589-99
LID - 10.1128/AEM.03154-09 [doi]
AB  - Bacteria in the genus Rickettsia are intracellular symbionts of disparate groups 
      of organisms. Some Rickettsia strains infect vertebrate animals and plants, where 
      they cause diseases, but most strains are vertically inherited symbionts of 
      invertebrates. In insects Rickettsia symbionts are known to have diverse effects 
      on hosts ranging from influencing host fitness to manipulating reproduction. Here 
      we provide evidence that a Rickettsia symbiont causes thelytokous parthenogenesis 
      (in which mothers produce only daughters from unfertilized eggs) in a parasitoid 
      wasp, Pnigalio soemius (Hymenoptera: Eulophidae). Feeding antibiotics to 
      thelytokous female wasps resulted in production of progeny that were almost all 
      males. Cloning and sequencing of a fragment of the 16S rRNA gene amplified with 
      universal primers, diagnostic PCR screening of symbiont lineages associated with 
      manipulation of reproduction, and fluorescence in situ hybridization (FISH) 
      revealed that Rickettsia is always associated with thelytokous P. soemius and 
      that no other bacteria that manipulate reproduction are present. Molecular 
      analyses and FISH showed that Rickettsia is distributed in the reproductive 
      tissues and is transovarially transmitted from mothers to offspring. Comparison 
      of antibiotic-treated females and untreated females showed that infection had no 
      cost. Phylogenetic analyses of 16S rRNA and gltA gene sequences placed the 
      symbiont of P. soemius in the bellii group and indicated that there have been two 
      separate origins of the parthenogenesis-inducing phenotype in the genus 
      Rickettsia. A possible route for evolution of induction of parthenogenesis in the 
      two distantly related Rickettsia lineages is discussed.
FAU - Giorgini, M
AU  - Giorgini M
AD  - Istituto per la Protezione delle Piante, CNR, via Universita 133, Portici (NA), 
      Italy 80055. giorgini@ipp.cnr.it
FAU - Bernardo, U
AU  - Bernardo U
FAU - Monti, M M
AU  - Monti MM
FAU - Nappo, A G
AU  - Nappo AG
FAU - Gebiola, M
AU  - Gebiola M
LA  - eng
SI  - GENBANK/EU881494
SI  - GENBANK/EU881495
SI  - GENBANK/EU881496
SI  - GENBANK/EU881497
SI  - GENBANK/EU881498
SI  - GENBANK/EU881499
SI  - GENBANK/EU881500
SI  - GENBANK/EU881501
SI  - GENBANK/EU881502
SI  - GENBANK/EU881503
SI  - GENBANK/EU881504
SI  - GENBANK/EU881505
SI  - GENBANK/EU881506
SI  - GENBANK/EU881507
SI  - GENBANK/EU881508
SI  - GENBANK/GU121643
SI  - GENBANK/GU121644
SI  - GENBANK/GU121645
SI  - GENBANK/GU121646
SI  - GENBANK/GU121647
SI  - GENBANK/GU121648
SI  - GENBANK/GU121649
SI  - GENBANK/GU121650
SI  - GENBANK/GU121651
SI  - GENBANK/GU121652
SI  - GENBANK/GU121653
SI  - GENBANK/GU121654
SI  - GENBANK/GU121655
SI  - GENBANK/GU121656
SI  - GENBANK/GU121657
SI  - GENBANK/GU121658
SI  - GENBANK/GU121659
SI  - GENBANK/GU121660
SI  - GENBANK/GU121661
SI  - GENBANK/GU121662
SI  - GENBANK/GU121663
SI  - GENBANK/GU121664
SI  - GENBANK/GU121665
SI  - GENBANK/GU121666
SI  - GENBANK/GU121667
SI  - GENBANK/GU121668
SI  - GENBANK/GU121669
SI  - GENBANK/GU559856
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100219
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/administration & dosage
MH  - Bacterial Proteins/genetics
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Genitalia/microbiology
MH  - Hymenoptera/*microbiology/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - *Parthenogenesis
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rickettsia/*physiology
MH  - Sequence Analysis, DNA
MH  - *Symbiosis
PMC - PMC2849191
EDAT- 2010/02/23 06:00
MHDA- 2010/06/16 06:00
PMCR- 2010/10/01
CRDT- 2010/02/23 06:00
PHST- 2010/02/23 06:00 [entrez]
PHST- 2010/02/23 06:00 [pubmed]
PHST- 2010/06/16 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - AEM.03154-09 [pii]
AID - 3154-09 [pii]
AID - 10.1128/AEM.03154-09 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2010 Apr;76(8):2589-99. doi: 10.1128/AEM.03154-09. Epub 
      2010 Feb 19.