PMID- 20173501
OWN - NLM
STAT- MEDLINE
DCOM- 20100524
LR  - 20221207
IS  - 1538-7151 (Electronic)
IS  - 0277-1691 (Linking)
VI  - 29
IP  - 2
DP  - 2010 Mar
TI  - Amplification of the human telomerase gene in liquid-based preparations is 
      associated with cervical dysplasia and carcinoma.
PG  - 157-64
LID - 10.1097/PGP.0b013e3181bb8034 [doi]
AB  - The aim of this study was to analyze the amplification of the human telomerase 
      gene (TERC) in cervical specimens by fluorescence in situ hybridization (FISH), 
      and FISH findings were compared with cytologic and histologic diagnoses. Slides 
      prepared from 123 liquid-based preparations from cervical specimens with 
      cytologic diagnoses of negative for squamous intraepithelial lesion or malignancy 
      (n=20), atypical squamous cells of undetermined significance (n=22), low-grade 
      squamous intraepithelial lesion (n=55), high-grade squamous intraepithelial 
      lesion (n=21), or invasive cervical carcinomas (n=5) were analyzed for the 
      amplification of TERC using a 2-color FISH probe. The results of the cytologic 
      analysis and those of concurrent or subsequent biopsies were compared with the 
      FISH findings. Results showed that amplification of TERC was significantly 
      associated with both cytologic and histologic diagnoses (P<0.05). Patients with 
      high-grade squamous intraepithelial lesion or squamous cell carcinoma cytology 
      diagnoses had significantly higher percentages of cells with the amplification of 
      TERC than did patients with low-grade squamous intraepithelial lesion, ASC-US, 
      and negative for squamous intraepithelial lesion or malignancy (P<0.005). FISH 
      can be performed on cervical liquid-based preparations to detect the 
      amplification of TERC. This test may be an adjunct to cytology screening, early 
      detection of cervix neoplasm, and may determine the progressive potential of 
      individual lesions, especially in high-risk patients.
FAU - Fan, Yi-Bo
AU  - Fan YB
AD  - Department of Pathology, The First Affiliated Hospital and College of Basic 
      Medical Sciences, China Medical University, Shenyang, China.
FAU - Wu, Xin
AU  - Wu X
FAU - Fu, Zhi-Min
AU  - Fu ZM
FAU - Wu, Guang-Ping
AU  - Wu GP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Gynecol Pathol
JT  - International journal of gynecological pathology : official journal of the 
      International Society of Gynecological Pathologists
JID - 8214845
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Female
MH  - Gene Amplification
MH  - Histocytochemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Telomerase/*genetics/metabolism
MH  - Uterine Cervical Dysplasia/enzymology/*genetics/pathology
MH  - Uterine Cervical Neoplasms/enzymology/*genetics/pathology
MH  - Young Adult
EDAT- 2010/02/23 06:00
MHDA- 2010/05/25 06:00
CRDT- 2010/02/23 06:00
PHST- 2010/02/23 06:00 [entrez]
PHST- 2010/02/23 06:00 [pubmed]
PHST- 2010/05/25 06:00 [medline]
AID - 00004347-201003000-00008 [pii]
AID - 10.1097/PGP.0b013e3181bb8034 [doi]
PST - ppublish
SO  - Int J Gynecol Pathol. 2010 Mar;29(2):157-64. doi: 10.1097/PGP.0b013e3181bb8034.