PMID- 20173956
OWN - NLM
STAT- MEDLINE
DCOM- 20100524
LR  - 20181023
IS  - 1094-4087 (Electronic)
IS  - 1094-4087 (Linking)
VI  - 18
IP  - 2
DP  - 2010 Jan 18
TI  - Fast STED microscopy with continuous wave fiber lasers.
PG  - 1302-9
LID - 10.1364/OE.18.001302 [doi]
AB  - We report on fast beam-scanning stimulated-emission-depletion (STED) microscopy 
      in the visible range using for resolution enhancement compact, low cost and 
      turn-key continuous wave (CW) fiber lasers emitting at 592 nm. Spatial 
      resolutions of 35 to 65 nm in the focal plane are shown for various samples 
      including fluorescent nanoparticles, immuno-stained cells with a non-exhaustive 
      selection of 5 commonly used organic fluorescent markers, and living cells 
      expressing the yellow fluorescent protein Citrine. The potential of the 
      straightforward combination of CW-STED and fast beam scanning is illustrated in a 
      movie of the endoplasmic reticulum (ER) of a living cell, composed of 100 frames 
      (6 microm x 12 microm), each of them acquired in a time shorter than 0.2 s.
FAU - Moneron, Gael
AU  - Moneron G
AD  - Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 
      Gottingen, Germany.
FAU - Medda, Rebecca
AU  - Medda R
FAU - Hein, Birka
AU  - Hein B
FAU - Giske, Arnold
AU  - Giske A
FAU - Westphal, Volker
AU  - Westphal V
FAU - Hell, Stefan W
AU  - Hell SW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Opt Express
JT  - Optics express
JID - 101137103
SB  - IM
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Fiber Optic Technology/*instrumentation
MH  - Image Enhancement/*instrumentation
MH  - *Lasers
MH  - Microscopy, Fluorescence/*instrumentation
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2010/02/23 06:00
MHDA- 2010/05/25 06:00
CRDT- 2010/02/23 06:00
PHST- 2010/02/23 06:00 [entrez]
PHST- 2010/02/23 06:00 [pubmed]
PHST- 2010/05/25 06:00 [medline]
AID - 194483 [pii]
AID - 10.1364/OE.18.001302 [doi]
PST - ppublish
SO  - Opt Express. 2010 Jan 18;18(2):1302-9. doi: 10.1364/OE.18.001302.