PMID- 20174860
OWN - NLM
STAT- MEDLINE
DCOM- 20101221
LR  - 20151119
IS  - 1573-6822 (Electronic)
IS  - 0742-2091 (Linking)
VI  - 26
IP  - 5
DP  - 2010 Oct
TI  - Cytogenetic biomonitoring of inhabitants of a large uranium mineralization area: 
      the municipalities of Monte Alegre, Prainha, and Alenquer, in the State of Para, 
      Brazil.
PG  - 403-19
LID - 10.1007/s10565-010-9152-8 [doi]
AB  - Uranium is a natural radioactive metallic element; its effect on the organism is 
      cumulative, and chronic exposure to this element can induce carcinogenesis. Three 
      cities of the Amazon region-Monte Alegre, Prainha, and Alenquer-in North Brazil, 
      are located in one of the largest uranium mineralization areas of the world. 
      Radon is a radioactive gas, part of uranium decay series and readily diffuses 
      through rock. In Monte Alegre, most of the houses are built of rocks removed from 
      the Earth's crust in the forest, where the uranium reserves lie. The objective of 
      the present work is to determine the presence or absence of genotoxicity and risk 
      of carcinogenesis induced by natural exposure to uranium and radon in the 
      populations of these three cities. The frequency of micronuclei (MN) and 
      chromosomal aberrations (CA) showed no statistically significant differences 
      between the control population and the three study populations (P > 0.05). MN was 
      also analyzed using the fluorescence in situ hybridization (FISH) technique, with 
      a centromere-specific probe. No clastogenic and/or aneugenic effects were found 
      in the populations. Using FISH analysis, other carcinogenesis biomarkers were 
      analyzed, but neither the presence of the IGH/BCL2 translocation nor an 
      amplification of the MYC gene and 22q21 region was detected. Clastogenicity and 
      DNA damage were also not found in the populations analyzed using the alkaline 
      comet assay. The mitotic index showed no cytotoxicity in the analyzed 
      individuals' lymphocytes. Once we do not have data concerning radiation doses 
      from other sources, such as cosmic rays, potassium, thorium, or anthropogenic 
      sources, it is hard to determine if uranium emissions in this geographic region 
      where our study population lives are too low to cause significant DNA damage. 
      Regardless, genetic analyses suggest that the radiation in our study area is not 
      high enough to induce DNA alterations or to interfere with mitotic apparatus 
      formation. It is also possible that damages caused by radiation doses undergo 
      cellular repair.
FAU - Guimaraes, Adriana Costa
AU  - Guimaraes AC
AD  - Institute of Biological Sciences, Federal University of Para, Belem, Para, 
      Brazil.
FAU - Antunes, Lusania Maria Greggi
AU  - Antunes LM
FAU - Ribeiro, Helem Ferreira
AU  - Ribeiro HF
FAU - dos Santos, Andrea Kelly Ribeiro
AU  - dos Santos AK
FAU - Cardoso, Plinio Cerqueira dos Santos
AU  - Cardoso PC
FAU - de Lima, Patricia Lima
AU  - de Lima PL
FAU - Seabra, Aline Damasceno
AU  - Seabra AD
FAU - Pontes, Thais Brilhante
AU  - Pontes TB
FAU - Pessoa, Claudia
AU  - Pessoa C
FAU - de Moraes, Manoel Odorico
AU  - de Moraes MO
FAU - Cavalcanti, Bruno Coelho
AU  - Cavalcanti BC
FAU - Sombra, Carla Maria Lima
AU  - Sombra CM
FAU - Bahia, Marcelo de Oliveira
AU  - Bahia Mde O
FAU - Burbano, Rommel Rodriguez
AU  - Burbano RR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100220
PL  - Switzerland
TA  - Cell Biol Toxicol
JT  - Cell biology and toxicology
JID - 8506639
RN  - 0 (Aneugens)
RN  - 0 (Carcinogens, Environmental)
RN  - 0 (Mutagens)
RN  - 0 (Radioactive Pollutants)
RN  - 0 (Soil Pollutants)
RN  - 4OC371KSTK (Uranium)
RN  - Q74S4N8N1G (Radon)
SB  - IM
MH  - Aneugens
MH  - Brazil
MH  - Carcinogens, Environmental
MH  - Case-Control Studies
MH  - *Chromosome Aberrations
MH  - Chromosome Breakage
MH  - Chromosome Segregation
MH  - Comet Assay
MH  - DNA Damage
MH  - *Environmental Exposure
MH  - Environmental Monitoring
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes
MH  - Micronucleus Tests
MH  - Mining
MH  - *Mutagenicity Tests
MH  - Mutagens/toxicity
MH  - *Radiation Dosage
MH  - Radioactive Pollutants/analysis
MH  - Radon/analysis
MH  - Soil Pollutants/analysis
MH  - Surveys and Questionnaires
MH  - Uranium/*toxicity
EDAT- 2010/02/23 06:00
MHDA- 2010/12/22 06:00
CRDT- 2010/02/23 06:00
PHST- 2009/05/18 00:00 [received]
PHST- 2010/01/20 00:00 [accepted]
PHST- 2010/02/23 06:00 [entrez]
PHST- 2010/02/23 06:00 [pubmed]
PHST- 2010/12/22 06:00 [medline]
AID - 10.1007/s10565-010-9152-8 [doi]
PST - ppublish
SO  - Cell Biol Toxicol. 2010 Oct;26(5):403-19. doi: 10.1007/s10565-010-9152-8. Epub 
      2010 Feb 20.