PMID- 20176923
OWN - NLM
STAT- MEDLINE
DCOM- 20100326
LR  - 20211020
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 188
IP  - 4
DP  - 2010 Feb 22
TI  - Condensin complexes regulate mitotic progression and interphase chromatin 
      structure in embryonic stem cells.
PG  - 491-503
LID - 10.1083/jcb.200908026 [doi]
AB  - In an RNA interference screen interrogating regulators of mouse embryonic stem 
      (ES) cell chromatin structure, we previously identified 62 genes required for ES 
      cell viability. Among these 62 genes were Smc2 and -4, which are core components 
      of the two mammalian condensin complexes. In this study, we show that for Smc2 
      and -4, as well as an additional 49 of the 62 genes, knockdown (KD) in somatic 
      cells had minimal effects on proliferation or viability. Upon KD, Smc2 and -4 
      exhibited two phenotypes that were unique to ES cells and unique among the ES 
      cell-lethal targets: metaphase arrest and greatly enlarged interphase nuclei. 
      Nuclear enlargement in condensin KD ES cells was caused by a defect in chromatin 
      compaction rather than changes in DNA content. The altered compaction coincided 
      with alterations in the abundance of several epigenetic modifications. These data 
      reveal a unique role for condensin complexes in interphase chromatin compaction 
      in ES cells.
FAU - Fazzio, Thomas G
AU  - Fazzio TG
AD  - Biochemistry and Biophysics Department, University of California, San Francisco, 
      San Francisco, CA 94158, USA. thomas.fazzio@ucsf.edu
FAU - Panning, Barbara
AU  - Panning B
LA  - eng
GR  - 1K99CA140854-01/CA/NCI NIH HHS/United States
GR  - R01 GM085186/GM/NIGMS NIH HHS/United States
GR  - R01 GM63671/GM/NIGMS NIH HHS/United States
GR  - K99 CA140854/CA/NCI NIH HHS/United States
GR  - T32CA09043/CA/NCI NIH HHS/United States
GR  - R01 GM063671/GM/NIGMS NIH HHS/United States
GR  - T32 CA009043/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Histones)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (condensin complexes)
RN  - 452VLY9402 (Serine)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
SB  - IM
MH  - Adenosine Triphosphatases/*metabolism
MH  - Anaphase
MH  - Animals
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Chromatin/*chemistry
MH  - DNA Damage
MH  - DNA-Binding Proteins/*metabolism
MH  - Embryo, Mammalian/cytology
MH  - Embryonic Stem Cells/*cytology/*metabolism
MH  - Fibroblasts/cytology/metabolism
MH  - Gene Knockdown Techniques
MH  - Histones/metabolism
MH  - *Interphase
MH  - Metaphase
MH  - Mice
MH  - *Mitosis
MH  - Multiprotein Complexes/*metabolism
MH  - Phenotype
MH  - Phosphorylation
MH  - RNA Interference
MH  - Serine/metabolism
MH  - Tumor Suppressor Protein p53/metabolism
PMC - PMC2828918
EDAT- 2010/02/24 06:00
MHDA- 2010/03/27 06:00
PMCR- 2010/08/22
CRDT- 2010/02/24 06:00
PHST- 2010/02/24 06:00 [entrez]
PHST- 2010/02/24 06:00 [pubmed]
PHST- 2010/03/27 06:00 [medline]
PHST- 2010/08/22 00:00 [pmc-release]
AID - jcb.200908026 [pii]
AID - 200908026 [pii]
AID - 10.1083/jcb.200908026 [doi]
PST - ppublish
SO  - J Cell Biol. 2010 Feb 22;188(4):491-503. doi: 10.1083/jcb.200908026.