PMID- 20178124
OWN - NLM
STAT- MEDLINE
DCOM- 20100719
LR  - 20100503
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 106
IP  - 3
DP  - 2010 Jun 15
TI  - Experimental evaluation of decrease in the activities of 
      polyphosphate/glycogen-accumulating organisms due to cell death and activity 
      decay in activated sludge.
PG  - 399-407
LID - 10.1002/bit.22703 [doi]
AB  - Decrease in bacterial activity (biomass decay) in activated sludge can result 
      from cell death (reduction in the amount of active bacteria) and activity decay 
      (reduction in the specific activity of active bacteria). The goal of this study 
      was to experimentally differentiate between cell death and activity decay as the 
      cause of decrease in bacterial activity. By means of measuring maximal anaerobic 
      phosphate release rates, verifying membrane integrity by live/dead staining and 
      verifying presence of 16S rRNA with fluorescence in situ hybridization (FISH), 
      the decay rates and death rates of polyphosphate-accumulating organisms (PAOs) in 
      a biological nutrient removal (BNR) system and a laboratory phosphate removing 
      sequencing batch reactor (SBR) system were determined, respectively, under famine 
      conditions. In addition, the decay rate and death rate of glycogen-accumulating 
      organisms (GAOs) in a SBR system with an enrichment culture of GAOs were also 
      measured under famine conditions. Hereto the maximal anaerobic volatile fatty 
      acid uptake rates, live/dead staining, and FISH were used. The experiments 
      revealed that in the BNR and enriched PAO-SBR systems, activity decay contributed 
      58% and 80% to the decreased activities of PAOs, and that cell death was 
      responsible for 42% and 20% of decreases in their respective activities. In the 
      enriched GAOs system, activity decay constituted a proportion of 74% of the 
      decreased activity of GAOs, and cell death only accounted for 26% of the decrease 
      of their activity.
CI  - 2010 Wiley Periodicals, Inc.
FAU - Hao, Xiaodi
AU  - Hao X
AD  - The R&D Centre for Sustainable Environmental Biotechnology, Beijing University of 
      Civil Engineering and Architecture, PR of China. xdhao@hotmail.com 
      <xdhao@hotmail.com>
FAU - Wang, Qilin
AU  - Wang Q
FAU - Cao, Yali
AU  - Cao Y
FAU - van Loosdrecht, Mark C M
AU  - van Loosdrecht MC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Polyphosphates)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Anaerobiosis
MH  - Bacteria/*metabolism
MH  - Bioreactors
MH  - *Cell Death
MH  - Cell Membrane/physiology
MH  - Glycogen/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - *Microbial Viability
MH  - Polyphosphates/*metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
MH  - Staining and Labeling
EDAT- 2010/02/24 06:00
MHDA- 2010/07/20 06:00
CRDT- 2010/02/24 06:00
PHST- 2010/02/24 06:00 [entrez]
PHST- 2010/02/24 06:00 [pubmed]
PHST- 2010/07/20 06:00 [medline]
AID - 10.1002/bit.22703 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2010 Jun 15;106(3):399-407. doi: 10.1002/bit.22703.