PMID- 20179890
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20171116
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 62
IP  - 1
DP  - 2010 Feb 25
TI  - The molecular mechanism of apoptosis of human umbilical vein endothelial cells 
      induced by monocyte chemotacitic protein-1.
PG  - 63-8
AB  - The present study was aimed to investigate whether Bcl-2, Fas and Bax are 
      involved in monocyte chemotacitic protein-1 (MCP-1)-induced apoptosis of human 
      umbilical vein endothelial cells (hUVECs). hUVECs were cultured, and the purity 
      was identified by immunofluorescence and immunohistochemistry with specific 
      anti-von Willebrand factor (vWF) and anti-VEGF receptor-2 (KDR) antibodies. With 
      90% confluence hUVECs were serum-starved for 12 h, and then treated with 
      different concentrations of MCP-1 (0.1, 1.0, 10, 100 ng/mL) for 24 and 48 h 
      respectively. The expressions of apoptosis related proteins Fas, Bcl-2, Bax were 
      detected by flow cytometry (FACS) and Western blot. As shown in our preliminary 
      study, MCP-1 induced apoptosis of hUVECs in a dose-dependent manner at both 24 h 
      and 48 h. FACS and Western blot analysis results in the present study indicated 
      that MCP-1 promoted the expression of proapoptotic proteins Bax and Fas and 
      inhibited the expression of antiapoptotic protein Bcl-2. These results suggest 
      that MCP-1 may induce the apoptosis of hUVECs through evoking the imbalance 
      between proapoptotic Fas/Bax and antiapoptotic Bcl-2 protein.
FAU - Li, Qin-Shan
AU  - Li QS
AD  - Department of Biochemistry and Molecular Biology, Zunyi Medical College, Zunyi 
      563003, China.
FAU - Liu, Yang
AU  - Liu Y
FAU - Feng, Zan-Jie
AU  - Feng ZJ
FAU - Lu, Zhi-Shun
AU  - Lu ZS
FAU - Qian, Min-Zhang
AU  - Qian MZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (BAX protein, human)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (FAS protein, human)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (fas Receptor)
SB  - IM
MH  - *Apoptosis
MH  - Atherosclerosis/*physiopathology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Human Umbilical Vein Endothelial Cells/*cytology
MH  - Humans
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - bcl-2-Associated X Protein/metabolism
MH  - fas Receptor/metabolism
EDAT- 2010/02/25 06:00
MHDA- 2013/01/25 06:00
CRDT- 2010/02/25 06:00
PHST- 2010/02/25 06:00 [entrez]
PHST- 2010/02/25 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
PST - ppublish
SO  - Sheng Li Xue Bao. 2010 Feb 25;62(1):63-8.